Amitava Sengupta

Dr. Amitava Sengupta

Senior Scientist

2013 Senior Scientist, Stem Cell & Leukemia Lab, CSIR-IICB, Kolkata
2012 Ramalingaswami Fellowship, Department of Biotechnology, Govt. of India
2007-12 The Institute of Cancer Research, UK/Lady Tata Memorial Trust International Postdoctoral Fellow, Stem Cell Program, Division of Experimental Hematology, Cincinnati Children’s Hospital Medical Center, OH, USA
2008 Ph.D., Biotechnology, Jadavpur University
2002 Post M.Sc., Biophysical Sciences, Saha Institute of Nuclear Physics, Kolkata
2001 M.Sc., Department of Biochemistry, University of Calcutta
1999 B.Sc., Chemistry, Rahara Vivekananda Centenary College, University of Calcutta

  • Stem cells possess two fundamental properties; self-renewal and differentiation. Bone marrow-resident adult hematopoietic stem cells (HSC) respond to physiological stimuli and regenerate hematopoiesis. Dysregulated self-renewal and arrest in differentiation of HSC and progenitors induce leukemic transformation. From a translational perspective HSCs draw attention because of their potential use in stem cell and gene therapy. We are interested at understanding the cell-autonomous and non-cell-autonomous molecular determinants that regulate HSC self-renewal, differentiation and interaction with hematopoietic microenvironment or niche.

    1. Cell-autonomous mechanisms of hematopoietic stem cell transformation.
    2. Epigenetic regulation of leukemia stem cell heterogeneity.
    3. Microenvironment regulation in hematopoiesis
  • Present Members
     
    Epigenetics & Leukemia Pathobiology Group
    Shankha Subhra Chatterjee (CSIR-SRF); M.Sc., Zoology, Banaras Hindu University; registered for PhD in Jadavpur University
    Mayukh Biswas (UGC-SRF); M.Sc., Biotechnology, St. Xavier’s College, Univ. of Calcutta; registered for PhD in Jadavpur University
     
    HSC Aging & Hematopoiesis Group
    Liberalis Debraj Boila (CSIR-SRF, SPM Fellow), M.Sc., Biotechnology, St. Xavier’s College, Univ. of Calcutta; registered for PhD in Jadavpur University
     
    Hematopoietic Microenvironment Group
    Sayantani Sinha (CSIR-SRF), M.Sc., Biotechnology, St. Xavier’s College, Univ. of Calcutta; registered for PhD in Jadavpur University
    Sayan Chakraborty (UGC-SRF); M.Sc., Molecular Biology, West Bengal Univ. of Technology; registered for PhD in Jadavpur University
     
    Alumni
    Subha Saha (DBT-RLS Project Assistant); M.Sc., Biochemistry, Univ. of Calcutta; presently pursuing PhD at ILS, Bhubaneswar
    Pranay Saha (DBT-RLS Project Assistant); M.Sc., Zoology, Univ. of Calcutta; presently pursuing PhD at IACS, Kolkata
    1. Chatterjee SS,Biswas M,1Boila LD, Banerjee D,Sengupta A*. SMARCB1 deficiency integrates epigenetic signals to oncogenic gene expression program maintenance in human acute myeloid leukemia. (*Corresponding author) Molecular Cancer Research (an official journal of AACR) 2018. http://mcr.aacrjournals.org/content/early/2018/04/02/1541-7786.MCR-17-0493.long
    2. Sinha S, Chatterjee SS, Biswas M, Nag A, Banerjee D, De R, Sengupta A*. SWI/SNF subunit expression heterogeneity in human aplastic anemia stem/progenitors. (*Corresponding author) Experimental Hematology(the official journal of ISEH)2018. http://www.exphem.org/article/S0301-472X(18)30163-2/fulltext
    3. Boila LD, Chatterjee SS, Banerjee D,Sengupta A*.KDM6 and KDM4 demethylases emerge as molecular therapeutic targets in human acute myeloid leukemia. (*Corresponding author) Experimental Hematology 2017 (the official journal of ISEH).https://www.ncbi.nlm.nih.gov/pubmed/29111428
    4. Chang KH,1Sengupta A,1Nayak R, Duran A, Lee SJ, Pratt R, Wellendorf AM, Hill SE, Watkins M, Gonzalez-Nieto D, Aronow BJ, Starczynowski DT, Civitelli R, Diaz-Meco MT, Moscat J, Cancelas JA. p62 is required for stem cell/progenitor retention through inhibition of IKK/NF-κB/Ccl4 signaling at the bone marrow, macrophage-osteoblast niche. (1Co-first author) Cell Reports 2014: 9, 2084-2097. https://www.ncbi.nlm.nih.gov/pubmed/25533346
    5. Taniguchi Ishikawa E, Chang KH, Nayak R, Olsson HA, Ficker A, Dunn SK, Madhu MN,Sengupta A,Whitsett JA, Grimes HL, Cancelas JA. Kruppel-like-factor 5 (Klf5) controls bone marrow lodging of hematopoetic stem cells and progenitors through Rab5-mediated active β1–integrin expression. Nature Communications 2013: 4, 1660 doi: 10.1038/ncomms2645. https://www.ncbi.nlm.nih.gov/pubmed/22498741
    1. Sengupta A,Ficker A, Dunn S, Madhu M, Cancelas JA. Bmi1 reprograms chronic myelogenous leukemia B-lymphoid progenitors to become B-ALL-initiating cells. Blood 2012: 119, 494-502. https://www.ncbi.nlm.nih.gov/pubmed/22101899
    1. Gonzalez-Nieto D, Li L, Kohler A, Ghiaur G, Ishikawa E,Sengupta A, Madhu M, Arnett J, Santho R, Dunn S, Fishman I, Gutstein D, Civitelli R, Barrio LC, Gunzer M, Cancelas JA. Connexin-43 in the osteogenic BM niche regulates its cellular composition and the bidirectional traffic of hematopoietic stem cells and progenitors. Blood 2012: 119, 5144-5154. https://www.ncbi.nlm.nih.gov/pubmed/22498741
    1. Chang KH, Sanchez-Aguilera A, Shen S,Sengupta A, Madhu M, Ficker A, Dunn S, Arnett J, Santo R, Agirre X, Perentesis J, Deininger M, Bustelo X, Williams DA, Cancelas JA.  Vav3 collaborates with p190-BCR-ABL in lymphoid progenitor leukemogenesis, proliferation and survival. Blood 2012: 120, 800-811. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3412345
    1. Sengupta A,Duran A, Ishikawa E, Florian M, Dunn S, Ficker A, Leitges M, Geiger H, Diaz-Meco MT, Moscat J, Cancelas JA. aPKCζ and aPKCλ are dispensable in hematopoietic stem cell activity and blood formation. Proc Natl Acad Sci USA 2011: 108, 9957-9962. https://www.ncbi.nlm.nih.gov/pubmed/21653884
    1. Sengupta A,Cancelas JA. A conundrum in mammalian hematopoietic stem cell polarity. Cell Cycle 2011: 10, 4191-4192. https://www.tandfonline.com/doi/abs/10.4161/cc.10.24.18361
    1. Sengupta A,Arnett JA, Dunn S, Williams DA, Cancelas JA. Rac2 GTPase deficiency depletes BCR-ABL+ leukemic stem cells and progenitors in vivoBlood 2010: 116, 81-84. https://www.ncbi.nlm.nih.gov/pubmed/20407032
    1. Ryan MA, Nattamai KJ, Xing E, Schleimer D, Daria D,Sengupta A,Kohler A, Liu W, Gunzer M, Jansen M, Ratner N, Le Cras TD, Waterstrat A, Van Zant G, Cancelas JA, Zheng Y, Geiger H. Pharmacological inhibition of EGFR signaling enhances G-CSF induced HSC mobilization. Nature Medicine 2010: 16, 1141-1146. https://www.ncbi.nlm.nih.gov/pubmed/20871610
    1. Sengupta A,Cancelas JA. Cancer Stem Cells: A stride towards cancer cure? J Cell Physiol 2010: 225, 7-14. https://www.ncbi.nlm.nih.gov/pubmed/20458736
    1. Sengupta A,Banerjee D, Chandra S, Banerji SK, Ghosh R, Roy R, Banerjee S. Deregulation and cross talk among Sonic hedgehog, Wnt, Hox and Notch signaling in chronic myeloid leukemia progression. Leukemia 2007: 21, 949-955. https://www.nature.com/articles/2404657
    1. Sengupta A,Banerjee S. Pleiotropic p27Kip1, BCR-ABL and Leukemic Stem Cell: The Trio in Concert. Leukemia 2007: 21, 2559-2561. https://www.nature.com/articles/2404842
    1. Sengupta A,Banerjee D, Chandra S, Banerjee S. Gene therapy for BCR-ABL+ human CML with dual phosphorylation resistant p27Kip1 and stable RNA interference using an EBV vector. J Gene Med 2006: 8, 1251-1261. https://www.ncbi.nlm.nih.gov/pubmed/16952195
    1. Chakrabarty M,Sengupta A, Bhattacharya D, Banerjee S, Chakrabarti A. DNA binding domain of RFX5: Interaction with X-box DNA and RFXANK.Biochim et Biophys Acta 2010: 1804, 2016-2024. https://www.ncbi.nlm.nih.gov/pubmed/20637319

    Book Chapter

    Sinha S, Boila LD, Chatterjee SS, Sengupta A*. miRNA and Cancer: A deadly liaison ? Cancer and Noncoding RNAs, (*Corresponding author) Elsevier 2017: 27-46.

    Selected Abstracts

    1. Sinha S,Sengupta A*. SWI/SNF: An important regulator of interaction between Hematopoietic stem/progenitors–Mesenchymal stromal microenvironment. Cold Spring Harbor Asia (CSHA) Conference on Chromatin, Epigenetics & Transcription,2018, Suzhou, China. (*Corresponding author, CSHA Travel Award).
    2. Biswas M,1Chatterjee SS,1 Boila LD, Banerjee D, Sengupta A*. Epigenetic plasticity in ATP-dependent chromatin remodeling complexes in human acute myeloid leukemia. (*Corresponding author, ASH Abstract Achievement AwardBlood 2017: 130, 3801. http://www.bloodjournal.org/content/130/Suppl_1/3801
    3. Biswas M, Chatterjee SS, Boila LD, Sinha S, Chakraborty S, Banerjee D,Sengupta A*. NuRD plasticity in human myelodysplasia/acute myeloid leukemia stem/progenitor cells.Keystone Symposia Conference Abstract: Hematopoiesis (B1), 2017, Fairmont Banff Springs, Banff, Alberta, Canada.
    4. Chatterjee SS, Biswas M, Boila LD, Sinha S, Chakraborty S, Banerjee D,Sengupta A*. SWI/SNF chromatin remodelers in human hematopoietic stem/progenitor cell activity. Keystone Symposia Conference Abstract: Chromatin & Epigenetics (C2), 2016, Whistler Conference Center, Whistler, British Columbia, Canada.
    5. Chang KH,Sengupta A,1Nayak R, Duran A, Lee SJ, Pratt R, Wellendorf A, Hill S, Watkins M, Civitelli R, Starczynowski DT, Diaz-Meco MT, Moscat J, Cancelas JA. Sqstm1 is required to retain hematopoietic stem cell/progenitors as a negative regulator of macrophage-dependent inflammatory signaling in the bone marrow osteoblastic niche. (1Equal contribution) Blood 2014: 124, 350. http://www.bloodjournal.org/content/124/21/350
    6. Taniguchi Ishikawa E, Chang KH, Olsson HA, Nayak RC, Ficker A, Dunn SK,Sengupta A, et al. Klf-5 controls HSC bone marrow homing and lodging through Rab5-mediated expression of active β1 integrin. Blood 2012: 120, 113. http://www.bloodjournal.org/content/120/21/113
    7. Sengupta A, Duran A, Gonzalez-Nieto D, Pratt R, Dunn S, Ficker A, Diaz-Meco MT, Moscat J, Cancelas JA. Sqstm1extrinsically regulates hematopoietic stem cell and progenitor mobilization. International Society for Stem Cell Research (ISSCR), 2011 Toronto, Canada.
    8. Sengupta A, Duran A, Ishikawa E, Florian M, Dunn S, Ficker A, Leitges M, Geiger H, Diaz-Meco MT, Moscat J, Cancelas JA. Hematopoietic stem cell activity and polarity are conserved in the absence of aPKCζ and aPKCλ.International Society for Stem Cell Research (ISSCR), 2011 Toronto, Canada.
    9. Sengupta A, Arnett JA, Dunn S, Cancelas JA. Bmi-1 overexpression synergizes with p210-BCR-ABL to induce stem cell and progenitor transformationin vitro and in vivoBlood 2009: 114, 3251. http://www.bloodjournal.org/content/114/22/3251
    1. Sengupta A, Arnett JA, Dunn S, Cancelas JA. Deletion of Rac2 GTPase inhibits proliferation of chronic myelogenous leukemia stem cells and progenitorsin vivo and promotes survival of Scl/p210-BCR-ABL mice. Blood 2009: 114, 3253. http://www.bloodjournal.org/content/114/22/3253
    2. Chang KH, Sanchez-Aguilera A,Sengupta A,Arnett J, Dunn S, Williams DA, Cancelas JA. Vav3 and Rac2 activation are required for B progenitor transformation induced by p190-BCR-ABL. Blood 2009: 114, 449. http://www.bloodjournal.org/content/114/22/449
    3. Gonzalez-Nieto D, Li L, Kohler A,Sengupta A,et al. Connexin-43 regulates the cell cycle entry of hematopoietic stem cells and the migration of hematopoietic progenitors towards and from bone marrow. Blood 2009: 114, 562. http://www.bloodjournal.org/content/114/22/562
    4. Sengupta A, Cancelas JA. Leukemic stem cells and progenitors demonstrate impaired interaction with the hematopoietic microenvironmentin vivo in an inducible murine model of chronic myelogenous leukemia. Blood 2008: 112, 191. http://www.bloodjournal.org/content/112/11/191
    5. Sengupta A, Cancelas JA. Leukemic progenitor retention in the bone marrow niche is impaired in an inducible murine model of chronic myelogenous leukemia.International Society of Experimental Hematology & Stem Cell Biology (ISEH), 2008 MA, USA.