Krishna Das Saha , Ph.D.

Principal Technical Officer
Cancer Biology & Inflammatory Disorder
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Research Focus

Inflammatory diseases, Anticancer activity, Nanoparticles, Leishmanial infection

Research Interest

My group is involved in evaluating (a) the disease ameliorating role of several natural and synthetic compounds and of different nanocomposites, (b) Modulation of immunobiological responses in leishmanial infection.

     (a) Compounds from herbal and dietary sources, synthetic origins are being examined to

  • Study the anticancer activity using in vitro and in vivo murine models like melanoma, sarcoma, hepatocarcinoma and colorectal carcinoma 
  • Evaluate their efficacy to improve the disorders of diseases like leishmaniasis, allergic asthma, septicaemia, liver damage, obesity, type I and type II diabetes, and inflammatory bowel disease 
  • Assess anti-oxidant, anti-inflammatory potency of the active compounds
  • Explore the mechanistic aspects of beneficial effects of  the active compounds
  • Formulate different organ specific improved targeted delivery vehicle of the active compounds/drugs with or without controlled release property
  • Develop specific targeted therapies of bioactive compounds or drugs combined with specific targeted diagnostics (Theranostics) 
  • Biological evaluation of delivery efficacy of the nanocomposites in vitro and in vivo systems

    (b) Leishmanial infection

  • Evaluation of antileishmanial activity of some natural compounds and different nanocomposites
  • Highlights on several imunnobiological changes in leishmanial infection 

Credentials

  •  Ph.D. on Immunology in 2002 from CSIR-IICB, Kolkata under Jadavpur University
  •  M.Sc. in Biochemistry in 1986 from University College of Science, Ballygunge of Calcutta University
  •  B.Sc. in Chemistry Hons in 1984 from Lady Brabourne College of Calcutta University

Honours & Awards

  • Technoloy Award from CSIR in 2002

Patents & Publications

PATENTS

  1. Bhadra, R., and Das. K.(1998). A process for the preparation of protozoan cell suspension useful as an immunomodulator for enhancement of host defense. 1679/Del /98.
  2. Bhadra, R., Pal B C, Das K. and Bhattacharya S. Active factor in Murraya koengii for relief, treatment and cure of asthmatic problem. PCT/IN/00102. 2000.
  3. Bhadra, R., Pal B C, Das K. and Bhattacharya S. Murraya koenigii extracts for treating asthma. PCT/IN2000/000102. 2002.
  4. Ranjan Bhadra, Bikash Chandra Pal, Krishna Das, Samir Bhattacharaya. Herbal composition for treating asthma. Application number: US 09/676,502.2004
  5. Bandyopadhyay S , Roy KC, Roy M, Pal BC, Bhadra R, Das K, Bhattacharya S. Herbal composition of blend of active components prepared from Murraya koengii and piper betle useful for blocking of 5 lipoxygenase activity leading to the inhibition of leukotriene synthesis, suppression of leukotriene synthesis, suppression of interleukin -4 production, and enhancement of gamma interferon release. 09/985,160, 11th January 2001.
  6. Bandyopadhyay S , Roy KC, Roy M, Pal BC, Bhadra R, Das K, Bhattacharya S. Herbal formulation of a combination of piper betel and Murraya koengii extracts for blocking 5-lipoxygenase activity. 09/925415, 8th Oct-2001

 

SELECTED PUBLICATIONS

  1. SK Das, S Mishra, K Manna, U Kayal, S Mahapatra, KD Saha, S Dalapati,  G. P. Das,  A.A. Mostafa  and  Asim Bhaumik; A new triazine based π-conjugated mesoporous 2D covalent organic framework: its in vitro anticancer activities. 2018, Chemical Communications54 (81), 11475-11478, doi 10.1039/c8cc07289b
  2. R Debnath, N Chatterjee, S Das, S Mishra, S Das, K Das Saha, D Ghosh, and D Maiti, Bromelain with peroxidase from pineapple are more potent to target leukemia growth inhibition - A comparison with only bromelain. 2018, Toxicol in Vitro, 17;55, 24-32,  doi10.1016/j.tiv.2018.11.004
  3. S Dey, R Purkait, C Patra , M Saha, S Mondal,  K Das Saha and C SinhaHighly selective and sensitive recognition of Zn(II) by a novel coumarinyl scaffold following spectrofluorometric technique and its application in living cells.2018, New Journal of Chemistry, 42, 16297-16306, doi10.1039/C8NJ03597K
  4. S Ghosh, SMallick; U Das; A Verma; U Pal; S Chatterjee; A Nandy; K Das Saha; N C Maiti; B  Baishya; G S Kumar; W  H GmeinerCurcumin stably interacts with DNA hairpin through minor groove binding and demonstrates enhanced cytotoxicity in combination with FdU nucleotides.2018, BBAGEN, 1862(3),485-494, doi 10.1016/j.bbagen.2017.10.018
  5. A K Bhanja, S Mishra, K Kar, K Naskar, S Maity, K Das Saha and C Sinha; Use of rhodamine-allyl Schiff base in chemodosimetric processes for total palladium estimation and application in live cell imaging.2018,New Journal of Chemistry, 42, 17351-17358, doi 10.1039/C8NJ04519D
  6. Biswadip Banerji*†‡ , Kadaiahgari Chandrasekhar†‡, Kancham Sreenath§, Saheli Roy∥, Sayoni Nag∥, and Krishna Das Saha∥; Synthesis of Triazole-Substituted Quinazoline Hybrids for Anticancer Activity and a Lead Compound as the EGFR Blocker and ROS Inducer Agent,2018, ACS OMEGA, 3 (11), 16134–16142,doi 10.1021/acsomega.8b01960
  7. Bhanja P, Mishra S, Manna K, Das Saha K, Bhaumik A.Porous Polymer Bearing Polyphenolic Organic Building Units as a Chemotherapeutic Agent for Cancer Treatment. 2018, ACS OMEGA, 3(1), 529-535, doi 10.1021/acsomega.7b01672
  8. Bhanja AK, Mishra S, Naskar K, Maity S, Das Saha K, Sinha C; Specific recognition of Cr3+ under physiological conditions by allyl substituted appendage rhodamine and its cell-imaging studies. 2017,Dalton Trans, 46(47), 16516-16524, doi 10.1039/c7dt03560h
  9. Bhanja P, Mishra S, Manna K, Mallick A, Das Saha K, Bhaumik A.Covalent Organic Framework Material Bearing Phloroglucinol Building Units as a Potent Anticancer Agent.2017, ACS Appl Mater Interfaces, 9(37),31411-31423,          doi 10.1021/acsami.7b07343
  10. Naskar B, Modak R, Maiti DK, Drew MGB, Bauzá A, Frontera A, Das Mukhopadhyay C, Mishra S, Das Saha K, Goswami S,A Schiff base platform: structures, sensing of Zn(ii) and PPi in aqueous medium and anticancer activity.2017 Dalton Trans, 46(28),9245-9252, doi 10.1039/c7dt01288h
  11. S. Saritha, K.C. Sunil,  U Das, S Mishra, M Sudarshan, K D Saha, S Dey, A Chakraborty, V Narayana, Das U, Kc S, Mishra S, Sudarshan M, Saha KD, Dey S, Chakraborty A, Narayana Y. Synthesis of a novel glucose capped gold nanoparticle as a better theranostic candidate, 2017, PLoS One, 12(6) e0178202doi 10.1371/journal.pone.0178202
  12. A K Bhanja, C Patra, S Mondal, S Mishra, K Das Saha, C Sinha.; Macrocycle aza-crown chromogenic reagent to Al3+ and fluorescence sensor for Zn2+ and Al3+ along with live cell application and logic operation. 2017, Sensors and Actuators B: Chemical, 252, 257–267, doi org/10.1016/j.snb.2017.05.178
  13. R  Nandi,  S Mishra,  T K Maji,  K Manna,  P  Kar,  S Banerjee,  S Dutta,  S. K. Sharma,  P  Lemmens,  K  D  Saha  and  S K PalA Novel Nanohybrid for Cancer Theranostics: Folate Sensitized Fe2O3 Nanoparticle for Colorectal Cancer Diagnosis and Photodynamic Therapy,2017, Journal of Materials Chemistry B, 5, 3927-3939, doi 10.1039/C6TB03292C
  14. A Nandy,  T Samanta,  S  Mallick,  P  Mitra,  S K Seth,  K D Saha,  S S Al-Deyabe  and  J  Dinda. Synthesis of Gold(III)←Gold(I)-NHC Through Disproportionation: R6289-6298 ole of Gold(I)-NHC in the induction of apoptosis in HepG2 cells, 2016, New Journal of Chemistry, 40,6289-6298 doi10.1039/C5NJ02979A
  15. C. Patra, A K. Bhanja, A. Mahapatra, S. Mishra and K. Das Saha, C. SinhaCoumarinyl thioether Schiff base as a turn-on fluorescent Zn(II) sensor and the complex as chemosensor for the selective recognition of ATP, along with its application in whole cell imaging2016RSC Adv676505-76513 doi10.1039/C6RA12369D
  16. Bose D, Banerjee S, Das S, Chatterjee N, Saha KD, Heat Killed Attenuated Leishmania Induces Apoptosis of HepG2 Cells Through ROS Mediated p53 Dependent Mitochondrial Pathway, 2016, Cell Physiol Biochem, 38(4), 1303-1318, doi10.1159/000443125
  17. Banerjee S, Bose D, Chatterjee N, Das S, Chakraborty S, Das T, Saha KD.; Attenuated Leishmania induce pro-inflammatory mediators and influence leishmanicidal activity by p38 MAPK dependent phagosome maturation in Leishmania donovani co-infected macrophages. 2016, Scientific report,6, 22335,doi 10.1038/srep22335
  18. Das S, Pradhan GK, Das S, Nath D, Das Saha KEnhanced protective activity of nano formulated andrographolide against arsenic induced liver damage. 2015,Chem Biol Interact, 242, 281-9,doi 10.1016/j.cbi.2015.10.011
  19. Das S, Chatterjee N, Bose D, Banerjee S, Jha T, Das Saha K.Antineoplastic impact of leishmanial sphingolipid in tumour growth with regulation of angiogenic event and inflammatory response. 2015, Apoptosis, 20(6),869-82,  doi 10.1007/s10495-015-1121-5.
  20. Das S, Chatterjee N, Bose D, Banerjee S, Jha T, Saha KD; Leishmanial sphingolipid induces apoptosis in Sarcoma 180 cancer cells through regulation of tumour growth via angiogenic switchover. 2015, Tumour Biol, 36(4), 3109-18, doi 10.1007/s13277-014-2947-0
  21. Bhunia S, Chatterjee N, Das S, Das Saha K, Bhaumik APorous polyurea network showing aggregation induced white light emission, applications as biosensor and scaffold for drug delivery.2014,  ACS Appl Mater Interfaces, 6(24),  22569-76,doi 10.1021/am5066859
  22. Chatterjee N, Das S, Bose D, Banerjee S, Jha T, Saha KD.; Leishmanial lipid suppresses the bacterial endotoxin-induced inflammatory response with attenuation of tissue injury in sepsis. 2014, J of leukoc Biol, 96(2), 325-36, doi 10.1189/jlb.3A1113-582R
  23. Nandy A, Dey SK, Das S, Munda RN, Dinda J, Saha KDGold (I) N-heterocyclic carbene complex inhibits mouse melanoma growth by p53 upregulation. 2014, Mol Cancer, 13, 57, doi10.1186/1476-4598-13-57
  24. Pramanik M, Chatterjee N, Das S, Saha KD, Bhaumik A.; Anthracene-bisphosphonate based novel fluorescent organic nanoparticles explored as apoptosis inducers of cancer cells. 2013, Chem Commun, 48(82), 9461-3,                       doi 10.1039/c3cc44989k
  25. Majumdar KN, Banerjee A, Ratha J, Mandal M, Sarkar RN, Saha KD. Leishmanial lipid suppresses tumor necrosis factor alpha, interleukin-1beta, and nitric oxide production by adherent synovial fluid mononuclear cells in rheumatoid arthritis patients and induces apoptosis through the mitochondrial-mediated pathway. 2008, Arthritis Rheum, 58(30), 696-706, doi 10.1002/art.23295