Nakul Chandra Maiti , Ph.D.

Senior Scientist
Structural Biology & Bioinformatics
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Research Focus

Structural and biochemical aspects of Intrinsically disordered proteins, Alzheimer’s , Parkinsons diseases, Biological Raman spectroscopy, Nanomaterials and its use in medicine

Research Interest

Our research focuses on two broad areas: (i) Intrinsically disordered protein, their link to different cellular activity and formation human diseases and  (II) to develope  porphyrin based nano-bio-composite for cancer therapy. Major methodologies that we employ in these invstigations include Raman/FTIR, NMR and Fluorescence and computational approaches. Many proteins or segments of it are intrinsically disordered (ID). It is estimated that about 50% of human proteins contain at least one long (> 30 residues) ID regions (IDR) and the number of IDR containing proteins is more in higher eukaryotes. Thus, it may have evolutionary importance. The lack of a rigid and folded stable structure in IDPs (intrinsically disorder proteins) may provide large plasticity to interact them efficiently with different targets, and thus conformational flexibility aids good efficacy in cell cycle regulation, membrane transport and different molecular recognition processes. In addition the inherited structural disorder  and the hydrophobic patches in it play an important role in the formation of protein assembly structure and the production of amyloid like aggregates that is implicated in several human neurological disorders. In our laboratory we attempt to define the role of this class of proteins in cell signalling and their implication in Alzheimer’s (AD) and Parkinson disease (PD). To address the role of protein oligomes  in AD and PD we are now studding different type of protein oligomers and membranes under Raman microscope. In addition, we are involved deeply in developing method that could aid in the early detection of AD and PD based on Raman signature. We are also studding the formation and possible structure of membrane less organelles which are unique as cellular storage for hormones and neurotransmitters. In addition Raman  spectroscopy  allow us to obtain the Raman spectrum  of very high quality of protein crystals.  We plan to probe the hydration and dynamics of the disordered region and side chain residues in some of the globular proteins in their crystalline form.  Further we will  define small molecule interaction, stability and structural evolution in the drug/protein complex formation inside single protein crystal. Our laboratory also investigate the interaction pattern of IDPs and structured proteins with metal nanoparticles.  Our results established that porphrin based nano-composite can significantly reduce the dose of doxorubicin and thereby showed improved efficacy towards killing of brain cancer cells.

Credentials

Academic Profile

  • Ph.D. Chemistry, TIFR, Mumbai 
  • M. Sc. Chemistry, University of Calcutta, Calcutta, India
  • B. Sc. Chemistry (honors), Presidency  College, University of Calcutta

 

Employment Details      

Grade/ Post

Estt./ Lab/ Instt.

 From

 To

Principal  Scientist

Indian Institute of Chemical Biology, Kolkata

May 2015

Present

Senior  Scientist

Indian Institute of Chemical Biology, Kolkata

May 2010

May 2015

Research Specialist

LSU-Agcenter, Baton Rouge, USA

Sept/ 2008

May/ 2010

Research Associate

California State University Los Angeles

June /2006

Aug/2008

Senior Research Associate

Case W. R. University, Cleveland, USA

Dec/2001

June/ 2006

Research associate

UMKC,  Kansas City, USA

June/ 2000

Dec/2000

JSPS visiting scientist (postdoctoral)

Inst. for Mole. Sci Japan

Jan/1998

June/2000

Honours & Awards

  • Raman Fellowship Award, 2012, CSIR, New Delhi, June 2012-Sept 2012
  • International JSPS fellowship award, Japan  Society for the  Promotion of Science, Japan, 1998-2000

 

Patents & Publications

SELECTED PUBLICATIONS

  1. Bera, Kaushik; Maiti, Samarpan; Maity, Mritunjoy; Mandal, Chitra; Maiti, Nakul C; Porphyrin–Gold Nanomaterial for Efficient Drug Delivery to Cancerous Cells. ACS Omega, 3, 4, 4602-4619, 2018 , ACS Publications
  2. Maity, Mritunjoy; Bera, Kaushik; Pal, Uttam; Khamaru, Krishnendu; Maiti, Nakul C; Sensing of Iron (III) Ion via Modulation of Redox Potential on Biliverdin Protected Silver Nanosurface. ACS Applied Nano Materials, 2018 , ACS Publications
  3. Mondal, Animesh; Pal, Uttam; Roy, Anupam; Maiti, Nakul C; Structural Intricacy of Disordered Regions in Transcription Factors Imparting Colon Cancer. Journal of Proteins & Proteomics, 9, 3, 2018 , Proteomics Society of India
  4. Dolui, Sandip; Roy, Anupam; Pal, Uttam; Saha, Achintya; Maiti, Nakul C; Structural Insight of Amyloidogenic Intermediates of Human Insulin. ACS Omega, 3, 2, 2452-2462, 2018 , ACS Publications
  5. Pal, Uttam; Maiti, Nakul Chandra; Pattern Based Detection of Potentially Druggable Binding Sites by Ligand Screening. Journal of Proteins & Proteomics, 9, 1, 2017 , Proteomics Society of India
  6. Roy, Anupam; Chandra, Kousik; Dolui, Sandip; Maiti, Nakul C; Envisaging the Structural Elevation in the Early Event of Oligomerization of Disordered Amyloid β Peptide. ACS Omega, 2, 8, 4316-4327, 2017 , ACS Publications
  7. Banerji, Biswadip; Chatterjee, Moumita; Pal, Uttam; Maiti, Nakul C; Formation of Annular Protofibrillar Assembly by Cysteine Tripeptide: Unraveling the Interactions with NMR, FTIR, and Molecular Dynamics. The Journal of Physical Chemistry B, 121, 26, 6367-6379, 2017 , ACS Publications
  8. Das, Swagata; Pal, Uttam; Maiti, Nakul Chandra; Metal ions provide structural stability and compactness to tetrameric purothionin. RSC Advances, 6, 93, 90690-90700, 2016 , Royal Society of Chemistry
  9. Banerji, Biswadip; Chatterjee, Moumita; Pal, Uttam; Maiti, Nakul Chandra; Molecular Details of Acetate Binding to a New Diamine Receptor by NMR and FT-IR Analyses. The Journal of Physical Chemistry A, 120, 15, 2330-2341, 2016 , ACS Publications
  10. Pal, Uttam; Roy, Anupam; Das, Supriya; Das, Swagata; Kundu, Mangaldeep; Bagga, Khyati; Maiti, Nakul Chandra; Origin of Protein Aggregation: Identification of Some Characteristic Traits in Structured and Intrinsically Disordered Proteins. Journal of Proteins & Proteomics, 7, 4, 2016 , Proteomics Society of India
  11. Das, Supriya; Pal, Uttam; Chatterjee, Moumita; Pramanik, Sumit Kumar; Banerji, Biswadip; Maiti, Nakul C; Envisaging Structural Insight of a Terminally Protected Proline Dipeptide by Raman Spectroscopy and Density Functional Theory Analyses. The Journal of Physical Chemistry A, 120, 49, 9829-9840, 2016 , ACS Publications
  12. Das, Swagata; Das, Supriya; Roy, Anupam; Pal, Uttam; Maiti, Nakul C; Orientation of tyrosine side chain in neurotoxic Aβ differs in two different secondary structures of the peptide. Royal Society Open Science, 3, 10, 160112, 2016 , The Royal Society
  13. Naiya, Gitashri; Raha, Paromita; Mondal, Manas Kumar; Pal, Uttam; Saha, Rajesh; Chaudhuri, Susobhan; Batabyal, Subrata; Pal, Samir Kumar; Bhattacharyya, Dhananjay; Maiti, Nakul C; Conformational selection underpins recognition of multiple DNA sequences by proteins and consequent functional actions. Physical Chemistry Chemical Physics, 18, 31, 21618-21628, 2016 , Royal Society of Chemistry
  14. Maity, Mritunjoy; Dolui, Sandip; Maiti, Nakul C; Hydrogen bonding plays a significant role in the binding of coomassie brilliant blue-R to hemoglobin: FT-IR, fluorescence and molecular dynamics studies. Physical Chemistry Chemical Physics, 17, 46, 31216-31227, 2015 , Royal Society of Chemistry
  15. Maiti, Nakul; Mondal, Payel; Das, Supriya; Role of Cyclophilin on Amyloid Formation and Protein Disaggregation. The FASEB Journal, 29, 1_supplement, 881.7, 2015 , The Federation of American Societies for Experimental Biology
  16. Pal, Uttam; Pramanik, Sumit Kumar; Bhattacharya, Baisali; Banerji, Biswadip; Maiti, Nakul Chandra; Binding interaction of a novel fluorophore with serum albumins: steady state fluorescence perturbation and molecular modeling analysis. Springer Plus, 4, 1, 548, 2015 , Nature Publishing Group
  17. Pal, Uttam; Sen, Sudeshna; Maiti, Nakul Chandra; Cα–H Carries Information of a Hydrogen Bond Involving the Geminal Hydroxyl Group: A Case Study with a Hydrogen-Bonded Complex of 1, 1, 1, 3, 3, 3-Hexafluoro-2-propanol and Tertiary Amines. The Journal of Physical Chemistry A, 118, 6, 1024-1030, 2014 , ACS Publications
  18. Maity, Mritunjoy; Das, Supriya; Maiti, Nakul C; Stability and binding interaction of bilirubin on a gold nano-surface: steady state fluorescence and FT-IR investigation. Physical Chemistry Chemical Physics, 16, 37, 20013-20022, 2014 , Royal Society of Chemistry
  19. Sen, Sudeshna; Pal, Uttam; Maiti, Nakul C; p K a Determination of d-Ribose by Raman Spectroscopy. The Journal of Physical Chemistry B, 118, 4, 909-914, 2014 , ACS Publications
  20. Das, Swagata; Pal, Uttam; Das, Supriya; Bagga, Khyati; Roy, Anupam; Mrigwani, Arpita; Maiti, Nakul C; Sequence complexity of amyloidogenic regions in intrinsically disordered human proteins. PloS One, 9, 3, e89781, 2014 , Public Library of Science
  21. Maity, Mritunjoy; Pramanik, Sumit Kumar; Pal, Uttam; Banerji, Biswadip; Maiti, Nakul Chandra; Copper (I) oxide nanoparticle and tryptophan as its biological conjugate: a modulation of cytotoxic effects. Journal of nanoparticle Research, 16, 1, 2179, 2014 , Springer
  22. Rudra, Deep Sankar; Pal, Uttam; Maiti, Nakul Chandra; Reiter, Russel J; Swarnakar, Snehasikta; Melatonin inhibits matrix metalloproteinase‐9 activity by binding to its active site. Journal of Pineal Research, 54, 4, 398-405, 2013 , Wiley Online Library
  23. Das, Supriya; Pal, Uttam; Das, Swagata; Maiti, Nakul C; Chaperone action of cyclophilin on lysozyme and its aggregate. Journal of Proteins & Proteomics, 4, 2, 2013 , Proteomics Society of India