Samit Chattopadhyay , FTWAS, FNA, FASc, FNASc, J C Bose National Fellow

Director, CSIR–IICB
Cancer Biology & Inflammatory Disorder
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Research Focus

Epigenetics, Cancer Biology, Immunology, Developmental Biology, Virology, Gene Regulation etc.

Research Interest

Introduction

The eukaryotic interphase chromatin is a highly organized structure. Specific scaffolding proteins form complexes with DNA and play pivotal role in DNA packaging. An important feature of DNA packaging involves folding of the chromatin into loop domains, which are periodically attached to the nuclear matrix through binding to specialized DNA sequences called Matrix Attachment Region or MARs. We study how proteins that specifically bind to MARs regulate genomic DNA organization and nuclear biochemical functions such as transcription, recombination, splicing, repair etc. Past several years our lab has been engaged in understanding the role of nuclear matrix and associated proteins in pathophysiological processes. We have focused on one such novel matrix associated protein SMAR1 that is down regulated in human breast cancer. It acts as a global repressor for many genes including Cyclin D1, IkBa and CK8 by directly recruiting HDAC1-mSin3a dependent repressor complex. Our findings reveal that SMAR1 functions in two different ways to regulate global gene expression. First, it acts as a transcriptional repressor and second, it modulates the transactivation potential of transcriptional co-activators NF-kB and p53. Additionally, NF-kB and p53 regulate various transcription factors involved in oncogenic transformation. These cofactors globally affect various signaling pathways leading to activation of genes that onset the process of tumorigenesis. We are therefore focusing our research work on understanding global gene regulation by SMAR1.

Topics of Research

  • Gene regulation and chromatin remodelling in cancer stem cells
  • Th1-Th2 differentiation of T cells
  • Regulation of splicing through nuclear matrix binding proteins
  • Epigenetic regulation during HIV transcription
  • Molecular mechanisms of DNA damage-repai

 

CURRENT PROJECTS

Role of SMAR1 in Cancer

Past several years our lab has been engaged in understanding the role of nuclear matrix binding proteins and their association with chromatin modifying complexes in pathophysiological and disease conditions. We have now characterized SMAR1 as a tumor suppressor by virtue of its ability to interact with tumor suppressor p53. We have also shown that SMAR1 delays tumor progression in mouse melanoma model by imposing cell cycle arrest. Additionally, we showed that the tumor suppressor function of SMAR1 resides within the RS domain that interacts with phosphorylated p53 and stabilizes it in the nucleus. Recently we have also shown that Cyclin D1 is a direct transcriptional target of SMAR1 and that SMAR1 is drastically reduced in breast cancer cell lines as well as in various grades of breast carcinoma tissues. SMAR1 regulates the cancer cell proliferation, and metastasis. We also find that chemotherapeutic agents like Doxorubicin induce the expression of SMAR1 in p53 dependent manner. We propose that SMAR1 acts as key regulator of cellular proliferation and metastasis in breast cancer by interplaying between p53 and TGFb pathway.

Role of SMAR1 in modulating cell cycle arrest and apoptosis

How tumor suppressor p53 bifurcates cell cycle arrest and apoptosis and executes these distinct pathways is not clearly understood. We find BAX and PUMA promoters harbor an identical MAR element and are transcriptional targets of SMAR1. Upon mild DNA damage, SMAR1 selectively represses BAX and PUMA through binding to the MAR independently of inducing p53 deacetylation through HDAC1. This generates an anti-apoptotic response leading to cell cycle arrest. Conversely, apoptotic DNA damage results in increased size and number of PML nuclear bodies with consequent sequestration of SMAR1. This facilitates p53 acetylation and restricts SMAR1 binding to BAX and PUMA MAR leading to apoptosis. Thus, our study establishes MAR as a damage responsive cis element and SMAR1-PML crosstalk as a switch that modulates the decision between cell cycle arrest and apoptosis in response to DNA damage. [EMBO,2009]

Regulation of NF-kB mediated transactivation by SMAR1

Regulation of NF-kB activity constitutes an important parameter for maintaining cellular homeostasis. Aberrant NF-kB activity as is seen in advanced grades of many tumors promotes the secretion of various cytokines and chemokines that help tumor cells to escape immunosurveillance and facilitate metastasis and angiogenesis. Therefore, inhibiting NF-kB activity is a major challenge to curb tumor growth. We have identified a distinct mechanism of NF-kB regulation by SMAR1 where we show that chemotherapeutic agents like Doxorubicin inhibit NF-kB mediated transactivation through SMAR1. The preliminary data suggest that SMAR1 can control specific subset of NF-kB target genes which promotes cancer growth, metastasis and angiogenesis.

Regulatory function of SMAR1 during IR induced DNA damage in cancer cells

Preliminary studies from lab suggest that SMAR1 is responsive to various stress stimuli. Therefore identification of stress responsive nature of this protein and delineating the signaling pathway that specifically stimulates the functions of this protein is of paramount importance. Utility of specific post-translational modifiers allows temporal and spatial control over protein relocalization and interactions, and may represent a means for trans-regulatory activation of protein activities. The ability to recognize these specific modifiers also underscores the capacity for signal amplification, a crucial step for the maintenance of genomic stability and tumor prevention. In context with the stress response, we identified a novel ATM phosphorylation site on SMAR1 that mediates an increased association of SMAR1 with Cyclin D1 promoter. The dual effect of SMAR1 i.e. induction of p21 through p53 activation and downregulation of Cyclin D1 by recruitment of corepressor complex causes cell cycle arrest.  SMAR1 is induced in response to any genotoxic insult. The studies are ongoing to decipher the roles of SMAR1 upon DNA damage and repair.

SMAR1 Represses HIV-1 LTR mediated transcription through chromatin remodeling

Nuclear Matrix and Matrix Attachment Regions (MARs) have been implicated in the transcriptional regulation of host as well as viral genes but their precise role in HIV-1 transcription remains unclear. Here, we show that >98% of HIV sequences in GenBank contain a consensus MAR element in their 5′ LTRs. We further define the role of this MAR in determining the state of viral transcription and show by MAR-binding assays that the transcriptionally silent HIV LTR has a strong propensity to bind to nuclear matrix. The MAR-binding protein SMAR1 aids in tethering LTR-MAR to nuclear matrix thereby enforcing transcriptional silencing. We have also characterized the minimal DNA sequence in the LTR-MAR to which SMAR1 binds and recruits the HDAC1/Sin3A corepressor complex thereby repressing LTR-mediated transcription (Virology, 2010)

Role of SMAR1 in T helper (TH) cell differentiation

The development and function of the T lymphocyte lineage are regulated tightly by signaling pathways that involve lineage-restricted cell surface receptors, intracellular signaling molecules and nuclear transcription factors. Naive T helper cells differentiate into two subsets, TH1 and TH2, each with distinct functions and their respective cytokine profiles. We have earlier shown that SMAR1 down regulates TH1 specific transcription factor T-bet thereby effecting TH1 lineage commitment of T cells. Using SMAR1 transgenic and knockout model we are trying to understand this differentiation process in detail.

Credentials

  • Director, Indian Institute of Chemical Biology, CSIR-IICB, Kolkata, 2015 August onwards
  • Founder Director, CSIR-IICB-TRUE Translation Research Unit of Excellence, Kolkata, 2016
  • Director, CSIR-NEIST, JORHAT (Assam) June 2018 onwards

 

Degree/ Diploma/ Certificate University/Institute Year Subjects
 Post Doctoral Research   Massachusetts Institute of   Technology  (MIT), Boston  1995-1998  Chromatin remodeling at TCRb locus during T development.   Role of cis elements in V(D)J recombination and allelic   exclusion using knock-out mouse model 
 Post Doctoral Research   University of Connecticut,   Farmington, USA   1989-  1995  Viral transcription, Transcription elongation through RNA-   protein interaction
 Doctorate  Jadavpur University, Kolkata, WB  1989  Ph D in Biochemistry, Jadavpur University, Calcutta (Physical   mapping of Vibrio. Cholera bacteriophage eltor-4 genome and   characterization of phage encoded tRNAs).
 Master’s Degree or Equivalent  University of Calcutta, WB  1984  Botany with Cytogenetics as special subject
 Bachelor’s degree  University of Calcutta, WB  1981  Botany Honours with Cytogenetics as special subject,   Zoology,  Human Physiology

Honours & Awards

  • Research Council Member, CCMB, Hyderabad, 2017-2020. 
  • Research Council Member, IMTECH, Chandigarh, 2017-2020. 
  • Convener, Sectional Committee, Indian National Science Academy (INSA) (2017 onwards)
  • Fellow of West Bengal Academy of Sciences (FAScT), 2016
  • Fellow of The World Academy of Sciences (TWAS), 2015
  • Convener, Guha Research Conference (GRC), 2014 
  • Sir J C Bose National Fellow, Department of Science and Technology, 2013
  • Fellow of Indian National Science Academy (FNA), Delhi, 2013
  • Associate Dean, Academic Cell, NCCS, Pune, 2012- 2015
  • Fellow of Academy of Science (FASc), Bangalore, 2011
  • Member of BIRAC, CRS, DBT, 2010 onwards
  • Member and examiner Thesis Committee, ACTREC, Navi Mumbai, 2007-2010
  • Fellow of National Academy of Science (FNASc), Allahabad, 2006
  • Member, Society of Indian Cell Biology, 2006
  • Co-Convener, Indian Society for Development Biologists (ISDB), 2004
  • Member, American Society for Biochemistry and Molecular Biology (ASBMB), USA. 2004
  • Member, Molecular Immunology Forum (MIF), 2002
  • Fellow of Maharastra Academy of Science (FMASc), 2000
  • Member of Research Advisory Board at Dr. D Y Patil Vidyapeeth, Pune
  • Member, Asian Transcription and Chromatin Biology, ChromatinAsia
  • Member, Indian Association for Cancer Research (ICAR)
  • Member, Scientific Advisory Committee (SAC), NII, New Delhi
  • Research Committee Member, CSIR, Animal Sciences and Biotechnology 
  • Task Force Member, CSIR, Inter-agency project IAP001, IICB, Kolkata
  • Task Force Member, DST-PAC, Delhi
  • Task Force Member: Cancer Biology, Department of Biotechnology, Delhi
  • Chairman/ Member, Ph D and project student selection committee at NCCS
  • Opted Member, DBT-JRF Fellowship, Government of India

 

Reviewer of several specialized journals like:

  • Virology
  • Journal of Biomedicine and Biotechnology
  • Cell Biology International, Elsevier
  • International Journal of Cancer
  • Journal Bioscience
  • International Journal of Biochemistry and Cell Biology
  • Cellular and Molecular Life Sciences
  • FEBS Journal
  • International Immunology (IMM)

Patents & Publications

PATENTS

  1. Chattopadhyay S and Jalota-Badhwar A., Tumor suppressor Activation polypeptide and uses thereof Indian patent file number: IPR/4.19.20/06083/2006. USA patent No. 8420100B2, April 16th, 2013 and  European patent, 2014 approved. 
  2. Samit Chattopadhyay, Nandaraj Taye, Prabhat Salunke. A modified peptide as an anti-cancer agent. Application number: WO2016139684A2/-PCT/IN2016/050081.
  3. Firoz Ali, Anila Hoeskere, Nandaraj Taye, Samit Chattopadhyay, Amitava Das. New reagent as scavenger of Hydrazine in drug induced cytotoxicity and in vitro enzymatic assay. Provisional Application No: 2332/DEL/2015
  4. Ramu Vadde, Nandaraj Taye, Samit Chattopadhyay, Amitava Das. Novel ruthenium (ii) complexes, preparation and uses thereof. Application number: US20160102357A1/ US14878568.
  5. Firoj Ali, Anila Hoskere, Nandaraj Taye, Samit Chattopadhyay, Amitava Das. Selective   determination of Free Cysteine. Provisional Application No: 2354/DEL/2015. 
  6. Anil Hoeskere, Nandaraj Taye, Amitava Das, Samit Chattophadhyay. An ER-Specific reagent for monitoring HNO in biological objects. Provisional Application No:   201611041925, Provisional filing date: 12/8/2016.
  7. Srivari Chandrasekhar, Togapur Pavan Kumar, Prathama S Mainkar Samit Chattopadhyay, Shruti Joshi, Chippala Venkataraju. Isothiocyanate compounds as SMAR1 stabilizers. Application File No: 201611028762. Filing Date: 24.08.201.

 

PUBLICATIONS

  1. Chattopadhyay S, Taye N, Alam A, Ghorai S, Chatterji DG, Parulekar A, Mogare D, Singh S, Sengupta P, Chatterjee S, Bhat MK, Santra MK, Salunkhe PB, Finston SK (2018) SMAR1 inhibits Wnt/β-catenin signaling and prevents colorectal cancer progression. Oncotarget. Apr 20;9(30): 21322-21336.
  2. Chattopadhyay S , Bhagat PN, Jadhav SH, , Paknikar KM (2018) Carbon nanospheres mediated nuclear delivery of SMAR1 protein (DNA binding domain) controls breast tumor in mice model. Nanomedicine (Lond). 2018 Feb;13(4):353-372.
  3. Mirlekar B, Gautam D, Chattopadhyay S. (2017) Chromatin Remodeling Protein SMAR1 Is a Critical Regulator of T Helper Cell Differentiation and Inflammatory Diseases. Frontiers in Immunology. 2017 Feb 9;8:72. doi: 10.3389/fimmu. 2017.00072. eCollection 2017. (IF: 5.7)
  4. Patel S, Choksi A, Pant R, Alam A and Chattopadhyay S. (2017) Nutritional programming of metabolic syndrome: Role of nutrients in shaping the epigenetics. A Handbook of nutrition, diet and epigenetics. Springer International Publishing AG 2017. DOI 10.1007/978-3-319-31143-2_42-1
  5. Paul D, Ghorai S., Chattopadhyay S., Dinesh US, Shetty P and Santra MK (2017) Cdc20 directs proteasome-mediated degradation of the tumor suppressor SMAR1 in higher grades of cancer through the anaphase promoting complex. Cell Death and Disease, NPG, In press. (IF: 5.4)
  6. Sengupta P, Chattopadhyay S, Chatterjee S., (2017) G-Quadruplex surveillance in BCL-2 gene: a promising therapeutic intervention in cancer treatment. Drug Discovery 2017 May 12. pii: S1359-6446(17)30245-3. doi: 10.1016/j.drudis.2017.05.001. (IF: 5.6)
  7. Chatterjee B, Banoth B, Mukherjee T, Taye N, Vijayaragavan B, Chattopadhyay S, Gomes J, Basak S. (2016) Delayed IBa synthesis insulates TLR4 induced canonical RelA/NFkB pathway from non-canonical LTbR signaling in myelomonocytic cells.  Science Signaling, AAAS, 2016 Dec 6;9 (457): ra120, (IF: 7.5)
  8. Ramu V, Aute S, Taye N, Guha R, Walker MG, Mogare D, Parulekar A, Thomas JA, Chattopadhyay S and Das A. (2017) Photo-induced cytotoxicity and anti-metastaticactivity of ruthenium(II)–polypyridyl complexesQ1 functionalized with tyrosine or tryptophan. Dalton Transaction. 2017 May 5. doi: 10.1039/c7dt00670e. [Epub ahead of print] (IF: 4.6)
  9. Agarwalla H, Mahajan PS, Sahu D, Taye N, Bishwajit Ganguly B, Mhaske SB, Chattopadhyay S, Das A. (2016) A Switch-On NIR Probe for Specific Detection of Hg2+ Ion in Aqueous Medium and in Mitochondria. Inorganic Chemistry. (IF: 4.82)
  10. Anila HA, Ali F, Kushwaha SA, Taye N, Chattopadhyay S, Das A. (2016) A Cysteine Specific Fluorescent Switch for Monitoring Oxidative Stress and Quantification of Aminoacylase-1 in Blood Serum. Analytical Chemistry. 2016 Dec 20; 88 (24): 12161-12168. doi: 10.1021/acs.analchem.6b03066. (IF: 5.7)
  11. Mathai J, Mittal SP, Alam A, Ranade P, Mogare D, Patel S, Saxena S, Ghorai S, Kulkarni AP, and Chattopadhyay S. (2016) SMAR1 binds to T(C/G) repeat and inhibits tumor progression by regulating miR-371-373 cluster. Scientific Reports (NPG) 2016 Sep 27;6:33779. doi: 10.1038/srep33779. PMID: 27671416. (IF: 5.3).
  12. Chemmannur SV, Bhagat P, Mirlekar B, Paknikar KM and Chattopadhyay S. (2016) Carbon nanospheres mediated delivery of nuclear matrix protein SMAR1 to direct experimental autoimmune encephalomyelitis in mice. International Journal of Nanomedicine (12;11:2039-51. doi: 10.2147/IJN.S93571. (IF: 4.4).
  13. Ali F, A AH, Taye N, Mogare DG, Chattopadhyay S, Das A. (2016) Specific receptor for hydrazine: mapping the in situ release of hydrazine in live cells and in an in vitro enzymatic assay. Chemical Communications (Cambridge). 2 (36): 6166-9. doi: 10.1039/c6cc01787h. Epub 2016 Apr 14. (IF: 6.8)
  14. Pal S, Ramu V, Taye N, Mogare DG, Yeware AM, Sarkar D, Reddy DS, Chattopadhyay S, Das A. (2016) GSH Induced Controlled Release of Levofloxacin from a Purpose-Built Prodrug: Luminescence Response for Probing the Drug Release in Escherichia coli and Staphylococcus aureus. Bioconjugate Chemistry. 21;27(9):2062-70. doi: 10.1021/acs.bioconjchem.6b00324. Epub 2016 Aug 26. (IF: 4.6).
  15. Yadav B, Malonia SK, Majumdar SS, Gupta P, Wadhwa N, Badhwar A, Gupta UD, Katoch VM, Chattopadhyay S. (2015) Constitutive expression of SMAR1 confers susceptibility to Mycobacterium tuberculosis infection in a transgenic mouse model. Indian Journal of Medical Research. 2015 Dec; 142(6):732-41. doi: 10.4103/0971-5916.174566. 
  16. Mirlekar B, Majumdar S, Khetmalas M, Chattopadhyay S. Regulation of T cell lineage commitment by SMAR1 during inflammatory & autoimmune diseases. (2015) Indian J Medical Res. 2015 Oct; 142(4):405-13. doi: 10.4103/0971-5916.169198.
  17. Nakka K, Chaudhary N, Joshi S, Bhat J, Chatterjee S, Mazumdar M, Adhikary A, Mukherjee S, De A, Santra M K, Das T and Chattopadhyay, S. (2015) ERK-MAPK signal-dependent and -independent regulation of alternative splicing by SMAR1 through Sam68 deacetylation. PNAS (30;112(26):E3374-83. doi: 10.1073/pnas.1418603112. (IF: 9.5).
  18. Padhye P, Alam A, Ghorai S, Chattopadhyay S, Poddar P. (2015) Doxorubicin-conjugated β-NaYF4:Gd(3+)/Tb(3+) multifunctional, phosphor nanorods: a multi-modal, luminescent, magnetic probe for simultaneous optical and magnetic resonance imaging and an excellent pH-triggered anti-cancer drug delivery nanovehicle. Nanoscale. 2015 Dec 14;7(46):19501-18. doi: 10.1039/c5nr04473a. Epub 2015 Nov 5. (IF: 7.5).
  19. Reddy G U, A AH, Ali F, Taye N, Chattopadhyay S, Das A. (2015) FRET-Based Probe for Monitoring pH Changes in Lipid-Dense Region of Hct116 Cells. Organic Letters. 2015 Nov 20;17(22):5532-5. doi: 10.1021/acs.orglett.5b02568. Epub 2015 Nov 2. (IF: 6.5).
  20. Khan D., Chattopadhyay S. and Das S. (2015) Influence of metabolic stress on translation of p53 isoforms. Molecular and Cellular Oncology, 015 May 5;3(1):e1039689. doi: 10.1080/23723556.
  21. Mirlekar B, Ghorai S., Khetmalas M and Chattopadhyay S. (2015) Nuclear Matrix Protein SMAR1 control regulatory T cell fate during Inflammatory Bowel Disease (IBD), Mucosal Immunology (Nature Publishing Group) 15 Nov; 8(6): 1184-200. (IF: 7.5).
  22. Chemmannur S, Badhwar A J, Mirlekar B, Malonia SK, Gupta M, Wadhwa N, Bopanna R, Mabalirajan U, Majumdar S, Ghosh B and Chattopadhyay S. (2015) A critical role of the nuclear MAR binding protein SMAR1 in lung homeostasis through the regulation of T cell differentiation. Mucosal Immunology (Nature Publishing Group) 2015 Nov; 8(6):1201-11. doi: 10.1038/mi. 2015.11. Epub 2015 Mar 4. (IF: 7.5).
  23. Chaudhary N, Nakka K K, Pavithra L, Bhat J, Chatterjee S and Chattopadhyay S (2014) SMAR1 coordinates HDAC6-induced deacetylation of Ku70 and dictates cell fate upon irradiation. Cell Death and Disease, (Nature Publishing Group) 2014 Oct 9, e1447; doi: 10.1038. (IF: 6.1).
  24. A AH, G UR, Ali F, Taye N, Chattopadhyay S, Das A. (2015) A reagent for specific recognition of cysteine in aqueous buffer and in natural milk: imaging studies, enzymatic reaction and analysis of whey protein. Chemical Communications (Camb). 2015 Nov 4;51(85):15592-5. doi: 10.1039/c5cc04876a. (IF: 6.8).
  25. Ali F, H A A, Taye N, Gonnade RG, Chattopadhyay S, Das A. A (2015) A fluorescent probe for specific detection of cysteine in the lipid dense region of cells. Chemical Communications (Camb). 2015 Dec 11;51(95):16932-5. doi: 10.1039/c5cc07450a. (IF: 6.8).
  26. Mirlekar B, Patil S, Bopanna R, Chattopadhyay S. (2015) MAR binding protein SMAR1 favors IL-10 mediated regulatory T cell function in acute colitis. Biochem Biophys Res Commun. 2015 Aug 21; 464 (2): 647-53. doi: 10.1016/j.bbrc.2015.07.028
  27. Khan D, Katoch Al, Das A, Lal R, Sharathchandra A, Chattopadhyay S and Das Saumitra (2015), Reversible induction of translational isoforms of p53 in glucose-deprivation Cell Death and Differentiation, (Nature Publishing Group) (In Press). (IF: 8.5).
  28. Ali F, Saha S, Maity A, Taye N, Si MK, Suresh E, Ganguly B, Chattopadhyay S, Das A. (2015) Specific Reagent for Cr(III): Imaging Cellular Uptake of Cr(III) in Hct116 Cells and Theoretical Rationalization. Journal of Physical Chemistry B. 2015 Oct 15; 119 (41): 13018-26. doi: 10.1021/acs.jpcb.5b07565. Epub 2015 Oct 2. (IF: 3.2).
  29. Reddy U G, Firoj Ali, Taye N, Chattopadhyay S and Das A (2015). A new turn on Pd2+-specific fluorescence probe and its use as an Imaging reagent for cellular uptake in Hct116 cells. Chemical Communications, (Cambridge) 2015 Feb 28;51(17):3649-52. doi: 10.1039/c4cc10171e. (IF: 6.8).
  30. Malonia SK., Yadav B, Sinha S, Lazennec G and Chattopadhyay S (2014) Chromatin remodeling protein SMAR1 regulates NF-B dependent Interleukin-8 (IL-8) transcription in breast cancer. The International Journal of Biochemistry and Cell Biology, 2014 Sep 18; 55C:220-226. doi: 10.1016/j.biocel.2014.09.008. [Epub ahead of print]. (IF: 4.5).
  31. Reddy G U, Ramu V., Roy S., Taye N., Chattopadhyay S. and Das A. (2014) A specific probe for Hg2+ to delineate even H+ in pure aqueous buffer / Hct116 colon cancer cells: Hg(II)-η2-arene π-interaction and a TBET-based fluorescence response. Chemical Communications (Cambridge), 2014 Oct 23; 50(92): 14421-4. (IF: 6.8).
  32. G UR, Agarwalla H, Taye N, Ghorai S, Chattopadhyay S, Das A. (2014) A novel fluorescence probe for estimation of Cysteine/ Histidine in human blood plasma and recognition of endogenous Cysteine in live Hct116 cells. Chemical Communications, (Cambridge) 2014 Jul 31; 50(69):9899-902. doi: 10.1039/c4cc04214j. (IF: 6.8).
  33. Chakraborty S, Das K, Saha S, Mazumdar M, Manna A, Chakraborty S, Mukherjee S, Khan P, Adhikary A, Mohanty S, Chattopadhyay S, Biswas S, Sa G, Das T. (2014) Nuclear matrix protein SMAR1 represses c-Fos-mediated HPV18 E6 transcription through alteration of chromatin histone de-acetylation.Journal of Biological Chemistry, Journal of Biological Chemistry 2014. Aug 25. pii: jbc.M114.564872. [Epub ahead of print] (IF: 4.6).
  34. Chakraborty S, Adhikary A, Mazumdar M, Mukherjee S, Bhattacharjee P, Guha D. Choudhuri T, Chattopadhyay S, Sa G, Sen A and Das T, (2014) Capsaicin-induced activation of p53-SMAR1 auto-regulatory loop down-regulates VEGF in non-small cell lung cancer to restrain angiogenesis. PLoS ONE, 13; 9 (6), 1-11. Wiley Publications (IF: 4.4).
  35. Mittal S P., Kulkarni A. P., Mathai J., Chattopadhyay S. and Pal J. K. (2014) Dose-dependent differential response of mammalian cells to cytoplasmic stress is mediated through the heme-regulated eIF2a kinase. International Journal of Biochemistry and Cell Biology 2014 Jul 31;54C:186-197. doi: 10.1016/j.biocel.2014.07.016. (IF: 4.5).
  36. Patel S, Choksi A and Chattopadhyay S (2014) Understanding Inter-individual Epigenetic Variations in Obesity and its Management. Personalized Epigenetics, Book chapter, Elsevier publication (In Press). 
  37. Chemmannur S and Chattopadhyay S (2014) Role of nuclear matrix associated region (MAR) binding proteins in the regulation of T helper cell differentiation. Proceedings of Indian National Science Academy, June 2 issue, 2014. 
  38. Adhikary A, Chakraborty S, Mazumdar M, Ghosh S, Mukherjee S, Manna A, Mohanty S, Nakka KK, Joshi S, De A, Chattopadhyay S, Sa G, Das T. (2014) Inhibition of Epithelial to Mesenchymal transition by E-cadherin up-regulation via repression of Slug transcription and inhibition of E-cadherin degradation: Dual role of SMAR1 in breast cancer cells. Journal of Biological Chemistry, Jul 30. 2014 pii: jbc.M113.527267. [Epub ahead of print] (IF: 4.6).
  39. Dhar A., Mallick S., Maiti A., Ghosh P.;F Ahmed, I., Bhattacharyya S., Mandal T., Manna A., Singh S., Nayak D., Wilder, P., Markowitz, J., Weber D., Ghosh M., Chattopadhyay S., Guha, R., Konar A., Bandyopadhyay S. and Roy S. (2014) Simultaneous inhibition of key growth pathways in melanoma cells and tumor regression by a designed bidentate constrained helical peptide. Biopolymers. 101 (4): 344-358. (IF: 3.0).
  40. Mittal S P, Mathai J, Kulkarni A P, Pal JK and Chattopadhyay S. (2013) miR-320a regulates erythroid differentiation through MAR binding protein SMAR1. International Journal of Biochemistry and Cell Biology, 45 (11):2519-2529. (IF: 4.5).
  41. Singh S, Raina V, Chavali P L, Dubash T, Kadreppa S, Parab P, Chattopadhyay S. (2012) Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment. BMC Molecular Biology. 1 ; 13:28. (IF: 3.5)
  42. Sinha S, Malonia SK, Mittal P K S, Pal J K and Chattopadhyay S. (2012) Tumor suppressor SMAR1 regulates p53 mediated transactivation by inhibiting acetyl transferase p300. International Journal of Biochemistry and Cell Biology, 44 (1): 46-52. (IF: 5.0).
  43. Malonia SK, Sinha S, Pavithra L, Singh K, Jalota-Bhadhwar A, Rampalli S, Kaul-Ghanekar R. and Chattopadhyay, S., (2011) Gene regulation by SMAR1 and its role as candidate tumor suppressor. BBA Reviews on Cancer, pp 1-12 (IF: 12.0). 
  44. Chaudhary N, Nakka KK, Maulik N. and Chattopadhyay S. (2011) Epigenetic Manifestation of Metabolic Syndrome and Dietary Management. Antioxidants and Redox Signaling, In Press (IF: 8.5).
  45. Sen GS, Mohanty S, Hossain DM, Bhattacharyya S, Banerjee S, Chakraborty J, Saha S, Ray P, Bhattacharjee P, Mandal D, Bhattacharya A, Chattopadhyay S, Das T and Sa G. (2011) Curcumin enhances the efficacy of chemotherapy by tailoring p65 NFB-p300 cross-talk in favor of p53-p300 in breast cancer. Journal of Biological Chemistry, 286 (49):42232-47. (IF: 6.0).
  46. Sinha S, Malonia SK, Mittal SPK, Singh K, Kadreppa S, Kamat R., Mukhopadhyaya R., Pal JK and  Chattopadhyay, S. (2010) Coordinated regulation of p53 apoptotic targets BAX and PUMA by SMAR1 through an identical MAR element, EMBO Journal. 29, 830-842.  (IF: 10.5)
  47. Singh S, Sreenath K, Pavithra L, Roy S, Chattopadhyay, S. (2010), SMAR1 regulates free radical stress through modulation of AKR1a4 enzyme activity. International Journal of Biochemistry and Cell Biology. 42 (7): 1105-14. (IF: 5.0)
  48. Sreenath K., Pavithra L., Singh S, Sinha S, Raut S, Dash PK., Siddappa NB., Mangaiarkarasi A, Ranga UK, Mitra D. and Chattopadhyay, S. (2010)  Nuclear Matrix Protein SMAR1 Represses HIV-1 LTR Mediated Transcription through Chromatin Remodeling. Virology, 25; 400(1): 76-85. (IF: 3.5)
  49. Pavithra L., Sreenath K., Singh S. and Chattopadhyay, S. (2010) Heat shock protein 70 binds to a novel sequence in 5’ UTR of tumor suppressor SMAR1 and regulates its mRNA stability upon PGA2 treatment. FEBS Letters, 19; 584(6): 1187-92. (IF: 3.0)
  50. Singh K., Sinha S, Malonia SK and Chattopadhyay, S. (2010) Tumor Necrosis Factor alpha (TNF-alpha) regulates CD40 expression through SMAR1 phosphorylation. BiochemIcal and Biophysical Research Communication. 8; 391(2): 1255-61. (IF: 3.0)
  51. Kopikar S., Choudhari A S., Kumari A., Chattopadhyay, S. and Kaul R (2010), Aqueous cinnamon extract (ACE-c) from the bark of Cinnamomum cassia causes apoptosis in human cervical cancer cell line (SiHa) through loss of mitochondrial membrane potential. BMC Cancer. 18; 10: 210. (IF: 3.0)
  52. Pavithra L, Chavali S. and Chattopadhyay, S. (2010) Nutritional epigenetics- impact on metabolic syndrome. Review in book chapter “Nutrition, Epigenetic Mechanisms and Human Disease”. CRC Press. Taylor and Francis Group, LLC, pp 259-286. 
  53. Chattopadhyay, S. Protein decides arrest or death. Nature News, (2010) doi: 10.1038/ nindia. 2010.65; Published online, May 24 issue.
  54. Singh K, Sinha S, Malonia SK, Bist P, Tergaonkar V, Chattopadhyay S. (2009) Tumor suppressor SMAR1 represses IBα expression and inhibits p65 transactivation through MARs. Journal of Biological Chemistry, 9; 284 (2):1267-78. (IF: 6.0)
  55. Pavithra L., Mukherjee S. Kadreppa S., Kar S., Sakaguchi K., Roy S. and Chattopadhyay S., (2009) SMAR1 forms ternary complex with p53-MDM2 and negatively regulates p53 mediated transcription. Journal of Molecular Biology, 388(4):691-702. (IF: 4.8)
  56. Pavithra L, Singh S, Sreenath K, Chattopadhyay S. (2009) Tumor suppressor SMAR1 downregulates Cytokeratin 8 expression by displacing p53 from its cognate site. International Journal of Biochemistry and Cell Biology. 41(4): 862-71. (IF: 5.0)
  57. Nakka K. and Chattopadhyay, S. (2009) Modulation of chromatin by MARs and MAR binding oncogenic transcription factor SMAR1. Molecular and Cellular Biochemistry, 336(1-2):75-84. (IF: 3.0)
  58. Singh V B, Pavithra L, Chattopadhyay S, Pal JK., (2008) Stress-induced overexpression of the heme-regulated eIF-2alpha kinase is regulated by Elk-1 activated through ERK pathway. Biochemical and Biophysical Research Communication. 379(3): 710 (IF: 3.0)
  59. Bavikar SN, Salunke DB, Hazra BG, Pore VS, Dodd RH, Thierry J, Shirazi F, Deshpande MV, Kadreppa S, Chattopadhyay S. (2008), Synthesis of chimeric tetrapeptide-linked cholic acid derivatives: impending synergistic agents. Bioorganic and Medicinal Chemistry Letters, 18 (20):5512-7. (IF: 3.0)
  60. Vatmurge NS, Hazra BG, Pore VS, Shirazi F, Deshpande MV, Kadreppa S, Chattopadhyay S, Gonnade RG. (2008) Synthesis and biological evaluation of bile acid dimers linked with 1, 2, 3-triazole and bis-beta-lactam. Organic and Biomolecular Chemistry. Oct 21; 6(20): 3823-30. (IF: 3.5)
  61. Pavithra L, Rampalli S, Sinha S., Sreenath K., Pestell R. G. and Chattopadhyay S. (2007) Stabilization of SMAR1 mRNA by PGA2 involves a stem loop structure in the 5' UTR. Nucleic Acids Research, 35: 6004-6016. (IF: 7.5)
  62. Singh, K. Mogare, D. Ramprasad O. G., Rajinikanth G., Pande, G. and Chattopadhyay, S. (2007) p53 Target gene SMAR1 is dysregulated in breast cancer: Its role in cancer cell migration and invasion. PLoS-ONE, 2(8): e660. (IF: 4.6)
  63. Pavithra, L and Chattopadhyay, S. Chromatin and cancer: Reprogramming chaos in the cell. (2007) National Academy of Science, 30 (3 and 4), 71-82 (Lead article). 
  64. Jalota-Badhwar, A., Kaul-Ghanekar, R., Mogare, D., Boppana, R., Packnikar, K. M. and Chattopadhyay, S. (2007) SMAR1-derived P44 peptide retains its tumor suppressor function through modulation of p53. Journal of Biological Chemistry, 282(13): 9902-13. (IF: 6.0)
  65. Chattopadhyay S and Pavithra L. MARs and MARBPs: key modulators of gene regulation and disease manifestation. (2006). Chromatin and Disease: Book, Vol 41, Series: Subcellular Biochemistry, Edited by Kundu and Dasgupta, Springer Publication. 
  66. Rampalli, S., Pavithra, L., Bhatt A., Tapas K. Kundu and Chattopadhyay, S. (2005). Tumor suppressor SMAR1 mediates Cyclin D1 repression by recruitment of SIN3/HDAC1 complex. Molecular and Cellular Biology, Vol. 25 October, (IF: 8.2)
  67. Kaul-Ghanekar, R., Majumdar, S., Jalota, A., Gulati, N., Dubey, N., Saha, B., Chattopadhyay, S. (2005) Abnormal V(D)J Recombination of T Cell Receptor {beta} Locus in SMAR1 Transgenic Mice. Journal of Biological Chemistry, 280 (10): 9450-9459. (IF: 6.5)
  68. Sarkar A, Kulkarni A, Chattopadhyay S, Mogare D, Sharma KK, Singh K, Pal JK. (2005) Lead-induced upregulation of the heme-regulated eukaryotic initiation factor 2 alpha kinase is compromised by hemin in human K562 cells. Biochem. Biophys. Acta. Dec 30; 1732(1-3):15-22. Epub 2005.
  69. Jalota A, Singh K, Pavithra L, Kaul R, Jameel S, Chattopadhyay S. (2005) Tumor suppressor SMAR1 activates and stabilizes p53 through its arginine-serine (RS) rich motif. Journal of Biological Chemistry, 280 (16), 16019-16029. (IF: 6.5)
  70. Kulkarni, A, Ravi, D. S., Singh, K., Rampalli, S., Parekh, V., Mitra, D., Chattopadhyay, S. (2005) HIV-1 Tat modulates T-bet expression and induces Th1 type of immune response Biochemical and Biophysical Research Communication. 329 (2): 706-712. (IF: 3.0)
  71. Kaul-Ghanekar, R., Jalota, A., L. Pavithra, Tucker, P. and Chattopadhyay, S. (2005) SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCR enhancer (E). Nucleic Acids Research, 32; 16: 4862-4875. (IF: 7.5)
  72. Kulkarni, A., Pavithra L, Rampalli, S., Mogare, D., Babu, K. Shiekh, G., Ghosh, S. and Chattopadhyay, S. (2005) HIV-1 integration sites are flanked by potential MARs that alone can act as promoters. Biochemical and Biophysical Research Communication, 322; 7672-77. 
  73. Kaul-Ghanekar, R., Jalota, A., L. Pavithra, Tucker, P. and Chattopadhyay, S. (2004) SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCR enhancer (E). Nucleic Acids Research, 32; 16: 4862-4875. (IF: 7.5)
  74. Kulkarni, A., Pavithra L, Rampalli, S., Mogare, D., Babu, K. Shiekh, G., Ghosh, S. and Chattopadhyay, S. (2004) HIV-1 integration sites are flanked by potential MARs that alone can act as promoters. Biochemical and Biophysical Research Communication, 322; 7672-77. (IF: 3.0)
  75. Kaul, R., Mukherjee, S., Ahmed, F., Bhat, M. K., Chhipa, R., Galande, S. and Chattopadhyay, S. (2003) Direct interaction and activation of p53 by SMAR1 causes cell cycle arrest at G2/M phase and delays tumor growth in mice. International Journal of Cancer, 103 (5), 606-615 (Cover page picture) (IF: 4.6)
  76. Rampalli, S., Kulkarni, A., Kumar, P., Mogare D., Galande, S., Mitra, D and Chattopadhyay, S. (2003) Stimulation of Tat independent transcriptional processivity from the HIV-1 LTR promoter by Matrix Attachment Regions. Nucleic Acids Research, 31, 3248-3256. (IF: 7.5)
  77. Chattopadhyay S. (2003) Anticancer protein identified, Nature News, September issue. 
  78. Prasad, D. V., Parekh, V. V., Banerjee, P. P., Chattopadhyay, S., Kumar, A. and Mishra, G. C. (2002) The Th1-specific costimulatory molecule, m150, is a post-translational isoform of lysozyme-associated membrane protein-1. Journal of Immunology, 169 (4); 1801-9. (IF: 7.1)
  79. Sarkar, A., Chattopadhyay, S., Kaul, R. and Pal, J. K. (2002) Lead exposure and heat shock inhibit cell proliferation in human HeLa and K562 cells by inducing expression and activity of the heme-regulated eIF-2-aKinase. Journal of Biochemistry, Molecular Biology & Biophysics, 6, 391-396. (IF: 3.0)
  80. Whitehurst, C., Chattopadhyay, S. and Chen, J. (2000) Control of V(D)J recombinational accessibility of the D1 gene segment at the TCR locus by a germline promoter.  Immunity, 10, 1-20. (IF: 21.0).
  81. Chattopadhyay S., Kaul R., Charest A., Houseman, D. and Chen, J.  SMAR1, a novel alternatively spliced gene product, binds to scaffold/ matrix associated region at TCR locus.  Genomics, 68, 93. 2000. (IF: 3.0)
  82. Chattopadhyay, S., Whitehurst, C., E. and Chen, J. (1998) A nuclear matrix attachment region (MAR) upstream of the T cell receptor gene enhancer binds Cux/ CDP and SATB1 and functions to repress transcription.  Journal of Biological Chemistry, 45, 29838-29846. (IF: 6.5) 
  83. Chattopadhyay, S., Whitehurst, C., E., Schwenk, F. and Chen, J.  (1998) Biochemical and functional analysis of chromatin changes of the T cell receptor gene locus during CD4-CD8- to CD4+CD8+ thymocyte differentiation.  Journal of Immunology, 160, 1256-1267. (IF: 7.5)
  84. Das, A., Pal, M., Garcia, M. J., Crossley, R., Whalen, W., Wolska, K., Byrd, R. A., Court, D., Costantino, N., Mazzula, M., Rees, W., von Hippel, P., Chattopadhyay, S., DeVito, J. and Ghosh, B., (1996) Components of a multiprotein-RNA complex that controls transcription elongation in E. coli phage lambda.  Methods in Enzymology, 274: pp 374-402. (IF: 4.0)
  85. Chattopadhyay, S., Hung, S. C., Das, A and Gottesman. M. E. (1995a) Interaction between the phage HK022 Nun protein and the nut RNA of phage lambda. PNAS, USA, 92:  pp12131-12135. (IF: 11.0)
  86. Chattopadhyay, S., Garcia, M.J. and Das, A. (1995b) Bipartite function of a small RNA hairpin in transcription anti-termination in bacteriophage lambda. PNAS, USA, 92: 4061-4065. (IF: 11.0) 
  87. Chattopadhyay, S., Garcia, J., Lazinski, D. and Das, A. (1991) Molecular basis of nut site recognition. Journal of Cellular Biochemistry, 15: 246. 
  88. Chattopadhyay, S. and Ghosh, R.K. (1989) The cloning and expression of transfer RNA gene cluster of Vibrio eltor  phage e4.  Virology, 171: 114.  (IF: 3.5)
  89. Nair, B., Oku,Y., Takeda,Y., Ghosh, A., Ghosh, R.K., Chattopadhyay, S., Pal, S. C., Kaper, B.J., Takeda, T. (1988) Toxin profiles of vibrio cholerae  Non-01 from environmental sources in Calcutta, India. Applied and Environmental Microbiology, 54: 3180. (IF: 4.2)
  90. Chattopadhyay, S. and Ghosh, R.K. (1988) Characterization of the transfer RNA coded by Vibrio eltor phage e4.  Virology, 165: 606. (IF: 3.5)
  91. Chattopadhyay, S. and Ghosh, R.K. (1988) Localization of the transfer RNA gene on the physical map of Vibrio eltor  phage e4.  Virology, 162: 337. (IF: 3.5)
  92. Chattopadhyay S, Kinchington, Ghosh RK (1987) Characterization of Vibrio eltor Typing Phages: Properties of the Eltor Phage e4. Journal of General Virology., May 1987 68: 1411-1416. (IF: 3.5).

 

BOOK CHAPTERS

  1. Patel S, Choksi A and Chattopadhyay S (2014) Understanding Inter-individual Epigenetic Variations in Obesity and its Management. Personalized Epigenetics, Book chapter, Elsevier publication (In Press). 
  2. Pavithra L, Chavali S. and Chattopadhyay S. (2010) Nutritional epigenetics- impact on metabolic syndrome. Review in book chapter on Molecular Mechanisms of Epigenetics. CRC Press. (2010), In press. 
  3. Chattopadhyay S and Pavithra L. (2006) MARs and MARBPs: key modulators of gene regulation and disease manifestation. Chromatin and Disease: Book, Vol 41, Series: Subcellular Biochemistry, Edited by Kundu and Dasgupta, Springer, 
  4. Pavithra, L and Chattopadhyay, S. (2007) Chromatin and cancer: Reprogramming chaos in the cell. National Academy of Science, 30 (3&4), 71-82. 
  5. Patel S, Choksi A and Chattopadhyay S (2014) Understanding Inter-individual Epigenetic Variations in Obesity and its Management. Personalized Epigenetics, Book chapter, Elsevier publication, 2015.
  6. Patel S, Choksi A, Pant R, Alam A and Chattopadhyay S. (2017) Nutritional programming of metabolic syndrome: Role of nutrients in shaping the epigenetics. A Handbook of nutrition, diet and epigenetics. Springer International Publishing AG 2017. DOI 10.1007/978-3-319-31143-2_42-1.