Paulomi Ghosh , Ph.D.
INSPIRE Faculty (DST)
Structural Biology & Bioinformatics
3D printing: Fused Deposition Modeling machines 3D print parts in a layer by layer fashion. A desired model of a scaffold is created using 3D CAD (Computer Aided Design) software. The native CAD file is exported to a STL (stereolithography file format) file. The STL file is then processed for orientation on the build plate, mathematical layer slicing, etc. The file is then sent to the 3D printer for scaffold fabrication. In a previous work, I have decellularized and incorporated cartilage extracellular matrix (ECM) in microspheres. The bioactive microspheres were then mixed with a suitable thermoplastic polymer and extruded to form filaments. The functionality of the encapsulated bioactive components was preserved during the extrusion process. The filaments containing microspheres were then used for producing a 3D scaffold by fused deposition modelling/3D printing. Further work on the ability of stem cells to differentiate on the 3D scaffold is ongoing.
Tissue Engineering: I have developed polymeric scaffolds such as micro/nano fibers, hydrogels, sponges, micro/nanospheres and performed their physico-chemical characterization and mechanical testing. The scaffolds were then seeded with mesenchymal stem cells to assess their influence on cell viability, proliferation and differentiation towards osteogenic/chondrogenic lineage. The scaffolds were subsequently implanted in animal models to study in vivo skeletal regeneration. I also developed a technology of fabricating living fibers wherein a polycationic polymer solution was extruded in a multivalent crosslinking bio-compatible bath containing living cells, pH 7.4, room temperature such that the fibers that were formed had a cell sheet on its circumference. The cell laden fibers (living fibers) may be used as a cell carrier for tissue engineering applications.
Medical Textile: Combination of medical science and textile technology has led to the emergence of medical textile industry. Applications of medical textile include wound dressing/gauze/surgical end-use/artificial ligament/hollow fibers for dialysis, etc. I have demonstrated the use of microfibers/nanofibers produced by wet spinning/electro-spinning for various tissue engineering applications.
Radiopaque polymer: I have developed a platform technology for generating radiopaque (X-ray opaque) polymer based derivatives via in situ iodination-cross-linking reactions for real time non-invasive imaging. I have demonstrated time-dependent movement of radiopaque microspheres through gastrointestinal tract in a rat model via delayed CT imaging. The opacity of the radiopaque derivatives was comparable to aluminum of equivalent thickness and commercially available radiopaque tape.
Ph.D., Indian Institute of Technology Kharagpur, India, 2010-15
Gandhian Young Technological Innovation Awardee, 2015
Winner and gold medallist of Department of Science and Technology -Lockheed Martin India Innovation Growth Program, 2015
Postdoctoral Research Fellow, Cincinnati Children`s Hospital Medical Center (CCHMC), USA, 2015-17
Patents & Publications
- Inventors: Paulomi Ghosh, Chia-Ying James Lin, Stacey Gruber, Patrick W. Whitlock.
Title of invention: Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold. US Patent, serial number: 62/578,037, Date of filing: 27/10/2017.
- Inventors: Paulomi Ghosh, Arun Prabhu Rameshbabu, Santanu Dhara.
Title of invention: Fiber-cell construct/tissue analogues comprising cell laden unit and process for manufacturing the same thereof. Application number: 278/KOL/2015, Date of Filing: 14/03/2015, Publication date: 1/12/2017.
- Inventors: Harpreet Singh, Nimmy Francis, Paulomi Ghosh, Santanu Dhara.
Title of invention: In situ iodination crosslinking of polymer formulations containing amine functionality for radioopacity, antimicrobial property obtained thereof. Application number: 1342/KOL/2014, Date of filing: 22/12/2014, Publication date: 26/08/2016
- Inventors: Paulomi Ghosh, Manisit Das, Santanu Dhara.
Title of invention: Chitosan based biodegradable materials for biomedical applications. Application number: 566/KOL/2013, Date of filing: 18/05/2013, Publication date: 21/11/2014.
- Paulomi Ghosh, Stacey M S Gruber, Chia-Ying Lin and Patrick W Whitlock. Microspheres containing decellularized cartilage induce chondrogenesis in vitro and remain functional after incorporation within a poly(caprolactone) filament useful for fabricating a 3D scaffold. Biofabrication 2018, 10, 025007.
- Paulomi Ghosh, Arun Prabhu Rameshbabu, Dipankar Das, Bhuvaneshwaran Subramanian, Sintu Kumar Samanta, Sabyasachi Roy, Sagar Pal, Sudip Kumar Ghosh and Santanu Dhara. Single-pot biofabrication of living fibers for tissue engineering applications. Journal of Materials Research 2018, 1.
- Arun Prabhu Rameshbabu, Kamakshi Bankoti, Sayanti Datta, Elavarasan Subramani, Anupam Apoorva, Paulomi Ghosh, Priti Prasanna Maity, Padmavati Manchikanti, Koel Chaudhury, and Santanu Dhara. Silk Sponges Ornamented with a Placenta-Derived Extracellular Matrix Augment Full-Thickness Cutaneous Wound Healing by Stimulating Neovascularization and Cellular Migration. ACS Appl. Mater. Interfaces 2018, 10 (20), pp 16977–16991.
- Dipankar Das, Arun Prabhu Rameshbabu, Paulomi Ghosh, Priyapratim Patra, Santanu Dhara, and Sagar Pal. Biocompatible nanogel derived from functionalized dextrin for targeted delivery of doxorubicin hydrochloride to MG 63 cancer cells. Carbohydrate Polymers 2017, 171, 27–38.
- Dipankar Das, Arun Prabhu Rameshbabu, Priyapratim Patra, Paulomi Ghosh, Santanu Dhara, and Sagar Pal. Biocompatible amphiphilic microgel derived from dextrin and poly (methyl methacrylate) for dual drugs carrier. Polymer, 2016, 107, 282–291.
- Nimmy K Francis, Harpreet Singh Pawar, Paulomi Ghosh, and Santanu Dhara. In situ iodination cross-linking of silk for radio-opaque antimicrobial surgical sutures. ACS Biomaterials Science & Engineering2016, 2, 188–196.
- Dipankar Das, Priyapratim Patra, Paulomi Ghosh, Arun Prabhu Rameshbabu, Santanu Dhara, and Sagar Pal. Dextrin and poly (lactide)-based biocompatible and biodegradable nanogel for cancer targeted delivery of doxorubicin hydrochloride. Polymer Chemistry, 2016, 7, 2965–2975.
- Arun Prabhu Rameshbabu, Paulomi Ghosh, Elavarasan Subramani, Kamakshi Bankoti, Kausik Kapat, Sayanti Datta, Priti Prasana Maity, Bhuvaneshwaran Subramanian, Sabyasachi Roy, Koel Chaudhury, and Santanu Dhara. Investigating the potential of human placenta-derived extracellular matrix sponges coupled with amniotic membrane-derived stem cells for osteochondral tissue engineering, Journal of Materials Chemistry B, 2016, 4, 613–625.
- Dipankar Das, Paulomi Ghosh, Animesh Ghosh, Chanchal Haldar, Santanu Dhara, Asit Baran Panda, and Sagar Pal. Stimulus-responsive, biodegradable, biocompatible, covalently cross-linked hydrogel based on dextrin and poly(N-isopropylacrylamide) for in vitro/in vivo controlled drug release, ACS Applied Materials & Interfaces, 2015, 7, 14338–14351
- Raghunath Das, Dipankar Das, Paulomi Ghosh, Santanu Dhara, Asit Baran Panda, and Sagar Pal. Development and application of a nanocomposite derived from crosslinked HPMC and Au nanoparticles for colon targeted drug delivery. RSC Advances, 2015, 5, 27481–27490.
- Paulomi Ghosh, Arun Prabhu Rameshbabu, Dipankar Das, Nimmy K Francis, Harpreet Singh Pawar, Bhuvaneshwaran Subramanian, Sagar Pal, and Santanu Dhara. Covalent cross- 5 Paulomi Ghosh, PhD links in polyampholytic chitosan fibers enhances bone regeneration in a rabbit model, Colloids and Surfaces B: Biointerfaces, 2015, 125, 160–169.
- Dipankar Das, Paulomi Ghosh, Santanu Dhara, Asit Baran Panda, and Sagar Pal. Dextrin and Poly(acrylic Acid) based biodegradable, non-Cytotoxic, chemically crosslinked hydrogel for Sustained Release of Ornidazole and Ciprofloxacin. ACS Applied Materials & Interfaces, 2015, 7, 4791–4803
- Paulomi Ghosh, Manisit Das, Arun Prabhu Rameshbabu, Dipankar Das, Sayanti Datta, Sagar Pal, Asit Baran Panda, and Santanu Dhara. Chitosan derivatives cross-linked with iodinated 2,5-dimethoxy-2,5-dihydrofuran for non-invasive imaging. ACS Applied Materials and Interfaces, 2014, 6, 17926–17936.
- Paulomi Ghosh, Arun Prabhu Rameshbabu, and Santanu Dhara. Citrate cross-linked gels with strain reversibility and viscoelastic behavior accelerate healing of osteochondral defects in a rabbit model. Langmuir, 2014, 30, 8442–8451.
- Paulomi Ghosh, Arun Prabhu Rameshbabu, Nantu Dogra, and Santanu Dhara. 2,5- Dimethoxy 2,5-dihydrofuran cross-linked chitosan fibers enhances bone regeneration in rabbit femur defects. RSC Advances, 2014, 4, 19516–19524.
- Arun Prabhu Rameshbabu, Saralasrita Mohanty, Kamakshi Bankoti, Paulomi Ghosh, and Santanu Dhara. Effect of alumina, silk and ceria short fibers in reinforcement of Bis- 8 Paulomi Ghosh, PhD GMA/TEGDMA dental resin. Composites Part B: Engineering, 2014, 70, 238–246.
- Raghunath Das, Dipankar Das, Paulomi Ghosh, Animesh Ghosh, and Santanu Dhara, Asit Baran Panda, Sagar Pal. Novel pH responsive graft copolymer based on HPMC and poly (acrylamide) synthesised by microwave irradiation: application in controlled release of ornidazole. Cellulose, 2014, 22, 313–327.
- Dipankar Das, Raghunath Das, Paulomi Ghosh, Santanu Dhara, Asit Baran Panda, and Sagar Pal. Dextrin Cross-linked with Poly (HEMA): A novel hydrogel for colon specific delivery of ornidazole. RSC Advances, 2013, 3, 25340–25350.
- Pallab Datta, Paulomi Ghosh, Kuntal Ghosh, Pritiprasanna Maity, Sintu Kumar Samanta, Sudip Kumar Ghosh, Pradeep Kumar Das Mohapatra, Jyotirmoy Chatterjee, and Santanu Dhara. In vitro ALP and osteocalcin gene expression analysis and In Vivo biocompatibility of N-Methylene phosphonic chitosan nanofibers for bone regeneration. Journal of Biomedical Nanotechnology, 2013, 9, 870–879.