Rupak K. Bhadra , Ph.D.

CSIR Emeritus Scientist
details-banner

Research Interest

  • Molecular basis of stringent response regulation in the cholera pathogen Vibrio cholerae cells under nutritional stress including detailed functional characterization of a newly discovered (p)ppGpp synthetase gene relV (at Author’s lab) and its role in the management of nutritional stress in V. cholerae.
  • Elucidation of the role of intracellular signaling molecule (p)ppGpp in regulation of gene expression related to growth, virulence, quorum sensing and biofilm formation.
  • Functional characterization of the RNA polymerase binding transcription factor DksA of V. cholerae
  • CTX phages, genome diversity and evolution of new clones of pathogenic V. cholerae.
  • Molecular studies on replication origins of V. cholerae and its use for various purposes.
  • Cold shock response and cold shock proteins of V. cholerae.

   

    Apart from above projects, the following projects have been initiated: 

  • Synthetic biology and metabolic engineering of commercially important plant secondary metabolites
  • Large scale sequencing of genomes and comparative genomics particularly bacterial genomics.

Credentials

PhD, Calcutta University, 1990

Patents & Publications

  • Kumar P, Bag S, Ghosh TS, Dey P, Dayal M, Saha B, Verma J, Pant A, Saxena S, Desigamani A, Rana P, Kumar D, Sharma NC, Hanpude P, Maiti TK, Mukhopadhyay AK, Bhadra RK, Nair GB, Ramamurthy T, Das B. Molecular Insights into Antimicrobial Resistance Traits of Multidrug Resistant Enteric Pathogens isolated from India. Sci Rep. 2017 Oct 31;7(1):14468. doi: 10.1038/s41598-017-14791-1.
  • Basu P, Pal RR, Dasgupta S, Bhadra RK. DksA-HapR-RpoS axis regulates haemagglutinin protease production in Vibrio cholerae. Microbiology. 2017 Jun;163(6):900-910. doi: 10.1099/mic.0.000469. Epub 2017 Jun 8.
  • Ghosh S, Mishra R, Biswas S, Bhadra RK, Mukhopadhyay PK. α-Lipoic Acid Mitigates Arsenic-Induced Hematological Abnormalities in Adult Male Rats. Iran J Med Sci. 2017 May;42(3):242-250.
  • Tapader R, Bose D, Basu P, Mondal M, Mondal A, Chatterjee NS, Dutta P, Basu S, Bhadra RK, Pal A. Role in proinflammatory response of YghJ, a secreted metalloprotease from neonatal septicemic Escherichia coli. Int J Med Microbiol. 2016 Nov;306(7):554-565. doi: 10.1016/j.ijmm.2016.06.003. Epub 2016 Jun 19.
  • Mondal R, Biswas S, Chatterjee A, Mishra R, Mukhopadhyay A, Bhadra RK, Mukhopadhyay PK. Protection against arsenic-induced hematological and hepatic anomalies by supplementation of vitamin C and vitamin E in adult male rats. J Basic Clin Physiol Pharmacol. 2016 Nov 1;27(6):643-652. doi: 10.1515/jbcpp-2016-0020.
  • Chowdhury G, Bhadra RK, Bag S, Pazhani GP, Das B, Basu P, Nagamani K, Nandy RK, Mukhopadhyay AK, Ramamurthy T. Rugose atypical Vibrio cholerae O1 El Tor responsible for 2009 cholera outbreak in India. J Med Microbiol. 2016 Oct;65(10):1130-1136. doi: 10.1099/jmm.0.000344. Epub 2016 Aug 25.
  • Ghosh R, Sharma NC, Halder K, Bhadra RK, Chowdhury G, Pazhani GP, Shinoda S, Mukhopadhyay AK, Nair GB, Ramamurthy T. Phenotypic and Genetic Heterogeneity in Vibrio cholerae O139 Isolated from Cholera Cases in Delhi, India during 2001-2006. Front Microbiol. 2016 Aug 9;7:1250. doi: 10.3389/fmicb.2016.01250. eCollection 2016.
  • Dasgupta S, Basu P, Pal RR, Bag S, Bhadra RK. Genetic and mutational characterization of the small alarmone synthetase gene relV of Vibrio cholerae. Microbiology-UK. doi: 10.1099/mic.0.079319-0. pp. 1-12. Epub ahead of print July 1, 2014.
  • Bag S, Das B, Dasgupta S, Bhadra RK. Mutational analysis of the (p)ppGpp synthetase activity of the Rel enzyme of Mycobacterium tuberculosis. Arch Microbiol. 196(8): 575-588. doi: 10.1007/s00203-014-0996-9. Epub ahead of print May 24, 2014.
  • Das B, Nair GB, Bhadra RK. Acquisition and dissemination mechanisms of CTXΦ in Vibrio cholerae : New paradigm for dif residents. World J Med Genetics. 4(2): 27-33, 2014.
  • Pal P, Pal A, Niyogi SK, Ramamurthy T, Bhadra RK. Comparative analysis of the genomes of Shigella dysenteriae type 2 & type 7 isolates. Indian J Med Res. 137(1):169-177, 2013.
  • Pal RR, Bag, S., Dasgupta, S., Das B., Bhadra, RK. Functional Characterization of the Stringent Response Regulatory Gene dksA of Vibrio cholerae and Its Role in Modulation of Virulence Phenotypes. J. Bacteriol. August 17, 2012 PMID22904284.
  • Bhadra, RK. 2012. Synthetic genetic circuits and its applications. Science & Culture. 78 (3-4): 122-125.
  • Pal RR, Das B, Dasgupta S, Bhadra RK. (2011) Genetic components of stringent response in Vibrio cholerae. Indian J Med Res. Feb;133(2):212-217.
  • Majumdar T, Das B, Bhadra RK, Dam B, Mazumder S. (2011) Complete nucleotide sequence of a quinolone resistance gene (qnrS2) carrying plasmid of Aeromonas hydrophila isolated from fish. Plasmid. 66(2): 79-84.
  • Syngkon A, Elluri S, Koley H, Rompikuntal PK, Saha DR, Chakrabarti MK, Bhadra RK, Wai SN, Pal A. (2010) Studies on a novel serine protease of a ΔhapAΔprtV Vibrio cholerae O1 strain and its role in hemorrhagic response in the rabbit ileal loop model. PLoS One. 5(9). pii: e13122
  • Bhadra, R. K., Das S., Nair, G. B. (2010) The Immunological basis for immunization Series: Module 14:Cholera. WHO, Geneva, pp. 1-43.
  • Halder, K., Das, B., Nair G. B., Bhadra R. K. (2010) Molecular evidence favouring step-wise evolution of Mozambique Vibrio cholerae O1 El Tor hybrid strain. Microbiology. 56: 99-107.
  • Das, B., Pal, R. R., Bag, S. and Bhadra, R. K. (2009) Stringent response in Vibrio cholerae: genetic analysis of spoT gene function and identification of a novel (p)ppGpp synthetase gene. Mol. Microbiol. 72: 380-398.
  • Chatterjee, S., Patra, T., Ghosh, K., Raychoudhuri, A., Pazhani, G. P., Das, M., Sarkar, B., Bhadra, R. K., Mukhopadhyay, A. K., Takeda, Y., Nair, G. B., Ramamurthy, T., Nandy, R. (2009) K. Vibrio cholerae O1 clinical strains isolated in 1992 in Kolkata with progenitor traits of the 2004 Mozambique variant. J. Med. Microbiol. 58: 239-247.
  • Bhowmick, R., Ghosal, A., Das, B., Koley, H., Saha, D. R., Ganguly, S., Nandy, R. K., Bhadra, R. K., Chatterjee, N. S. Intestinal adherence of Vibrio cholerae involves a coordinated interaction between colonization factor GbpA and mucin. Infect. Immun. 76: 4968-4977. 2008.
  • Shah, S., Das, B. and Bhadra, R. K. (2008) Functional analysis of the essential GTP-binding protein coding gene cgtA of Vibrio cholerae. J. Bacteriol. 190: 4764-4771.
➤