Bijesh P., , Ph.D.

Scientist
Cell Biology & Physiology
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Research Interest

One carbon metabolism (1C) involves an array of metabolic pathways that include methionine and folate cycles. The methyl groups are transferred in 1C that are involved in the synthesis of purines and pyrimidines, homeostasis of amino acids such as glycine, serine, and methionine, and epigenetics. Mitochondrial 1C is an important area, especially concerning geriatric diseases. 

Although the role of 1C in developing cells/tissues is well elucidated, its role in adult non-proliferative tissues is yet to be studied in deep. Our work focuses on downstream pathways involving 1C and its cross-talk with various disorders. Our primary focus is on 1C metabolism in progressive lung disorders such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD).

Figure: Importance of one-carbon metabolism in cellular homoeostasis. One carbon metabolism can be targeted for regenerating lung alveolar structure as it is highly active in the alveolar epithelial type 2 cells.

Credentials

  • Post-Doctoral Research Associate: University of Texas Health Science Center, Tyler, USA
  • Ph.D. in Biotechnology: CSIR-Central Food Technological Research Institute, CSIR-CFTRI, Mysuru, Karnataka, (Degree from University of Mysore) 

 

Honours & Awards

  • CSIR JRF 
  • Graduate Aptitude Test in Engineering (GATE) 
  • Second Rank at University in M.Sc. (2007)

 

Grants & Supports

  • Targeting FOLR1 for treating progressive inflammatory lung disorders such as COPD. SERB Department of Science & Technology (DST), 2023-2025, Rs 30.57 Lakhs, Startup Research Grant (SRG/2023/000091).

Patents & Publications

Publications:

 

  1. Das, D. N., Puthusseri Bijesh, Gopu, V., Krishnan, V., Bhagavath, A. K., Bolla, S., ... & Shetty, S. (2023). Caveolin-1-derived peptide attenuates cigarette smoke-induced airway and alveolar epithelial injury. American Journal of Physiology-Lung Cellular and Molecular Physiology, 325(5), L689-L708.
  2. Saad, K. R., Kumar, G., Puthusseri Bijesh, Srinivasa, S. M., Giridhar, P., & Shetty, N. P. (2023). Genome-wide identification of MATE, functional analysis and molecular dynamics of DcMATE21 involved in anthocyanin accumulation in Daucus carota. Phytochemistry, 210, 113676.
  3. Peethambaran, D., Puthusseri Bijesh, Kumar, G., Janani, R., Giridhar, P., & Baskaran, V. (2023). miR-7-5p Antagomir Protects Against Inflammation-Mediated Apoptosis and Lung Injury via Targeting Raf-1 In Vitro and In Vivo. Inflammation, 46(3), 941-962.
  4. Pathikkal, A., Puthusseri Bijesh, Divya, P., Rudrappa, S., & Chauhan, V. S. (2022). Folate derivatives, 5-methyltetrahydrofolate and 10-formyltetrahydrofolate, protect BEAS-2B cells from high glucose–induced oxidative stress and inflammation. In Vitro Cellular & Developmental Biology-Animal, 58(5), 419-428.
  5. Kumar, G., Arya, M., Padma, R., Puthusseri Bijesh, & Giridhar, P. (2022). Distinct GmASMTs are involved in regulating transcription factors and signalling cross-talk across embryo development, biotic, and abiotic stress in soybean. Frontiers in Plant Science, 13, 948901.
  6. Kumar, G., Saad, K. R., Arya, M., Puthusseri Bijesh, Mahadevappa, P., Shetty, N. P., & Giridhar, P. (2021). The Synergistic role of serotonin and melatonin during temperature stress in promoting cell division, ethylene and isoflavones biosynthesis in Glycine max. Current Plant Biology, 26, 100206.
  7. Kumar, G., Saad, K. R., Puthusseri Bijesh, Arya, M., Shetty, N. P., & Giridhar, P. (2021). Exogenous serotonin and melatonin regulate dietary isoflavones profoundly through ethylene biosynthesis in soybean [Glycine max (L.) Merr.]. Journal of Agricultural and Food Chemistry, 69(6), 1888-1899.
  8. Dorairaj, D., Puthusseri Bijesh, & Shetty, N. P. (2020). Suppression of N-glycan processing enzymes by deoxynojirimycin in tomato (Solanum lycopersicum) fruit. 3 Biotech, 10, 1-12. ISSN: 2190-5738
  9. Lokesh, V., Manjunatha, G., Hegde, N. S., Bulle, M., Puthusseri Bijesh, Gupta, K. J., & Neelwarne, B. (2019). Polyamine induction in postharvest banana fruits in response to NO donor SNP occurs via L-arginine mediated pathway and not via competitive diversion of S-adenosyl-l-methionine. Antioxidants, 8(9), 358. ISSN: 2076-3921
  10. Mallappa, M., Savanur, M. A., Gowda, B. G., Vishwanth, R. S., & Puthusseri Bijesh. (2019). Molecular Interaction of Hemorrheologic Agent, Pentoxifylline with Bovine Serum Albumin: An Approach to Investigate the Drug Protein Interaction Using multispectroscopic, Voltammetry and Molecular Modelling Techniques. Zeitschrift für Physikalische Chemie, 233(7), 973-994. ISSN: 2196-7156
  11. Puthusseri Bijesh., Divya, P., Lokesh, V., Kumar, G., Savanur, M. A., & Neelwarne, B. (2018). Novel Folate Binding Protein in Arabidopsis Expressed during Salicylic Acid-Induced Folate Accumulation. Journal of agricultural and food chemistry, 66(2), 505-511. 
  12. Puthusseri Bijesh., Divya, P., Veeresh, L., Kumar, G., & Neelwarne, B. (2018). Evaluation of folate-binding proteins and stability of folates in plant foliages. Food chemistry, 242, 555-559. 
  13. Puthusseri Bijesh., Marudamuthu, A., Tiwari, N., Fu, J., Idell, S., & Shetty, S. (2017). Regulation of p53-mediated changes in the uPA-fibrinolytic system and in lung injury by loss of surfactant protein C expression in alveolar epithelial cells. American Journal of Physiology-Lung Cellular and Molecular Physiology, 312(6), L783-L796. https://doi.org/10.1152/ajplung.00291.2016. 
  14. Puthusseri Bijesh., Divya, P., Lokesh, V., & Neelwarne, B. (2013). Salicylic acid-induced elicitation of folates in coriander (Coriandrum sativum L.) improves bioaccessibility and reduces pro-oxidant status. Food Chemistry. 136, 569–575. doi:10.1016/j.foodchem.2012.09.005. 
  15. Puthusseri Bijesh., Divya, P., Lokesh, V., & Neelwarne, B. (2012). Enhancement of folate content and its stability using food grade elicitors in coriander (Coriandrum sativum L.). Plant Foods for Human Nutrition, 67(2), 162–170. doi:10.1007/s11130-012-0285-1. 
  16. Puthusseri Bijesh., Smina, T. P., Janardhanan, K. K., & Manimohan, P. (2010). Antioxidant and anti-inflammatory properties of new medicinal fungus, Auriculoscypha anacardiicola D. A. Reid et Manim. (Agaricomycetideae), from India. International Journal of Medicinal Mushrooms, 12(4), 391–400. doi:10.1615/IntJMedMushr.v12.i4.60. 
  17. Divya, P., Puthusseri Bijesh., Savanur, M. A., Lokesh, V., & Neelwarne, B. (2018). Effects of methyl jasmonate and carotenogenic inhibitors on gene expression and carotenoid accumulation in coriander (Coriandrum sativum L.) foliage. Food Research International, 111, 11-19. 
  18. Gaonkar, S., Savanur, M. A., Sunagar, M. G., Puthusseri Bijesh., Deshapande, N., Nadaf, A. A., & Khazi, I. A. M. (2018). Exploring the potential of newly synthesized 4-methyl-6-morpholino-pyrimidine derivatives as antiproliferative agents. New Journal of Chemistry, 42(4), 2790-2803. DOI.10.1039/C7NJ04157H. 
  19. Divya, P., Puthusseri Bijesh., & Neelwarne, B. (2014). The effect of plant regulators on the concentration of carotenoids and phenolic compounds in foliage of coriander. LWT-Food Science and Technology, 56(1), 101–110. http://dx.doi.org/10.1016/j.lwt.2013.11.012. 
  20. Lokesh, V., Divya, P., Puthusseri Bijesh., Manjunatha, G., & Neelwarne, B. (2014). Profiles of carotenoids during post-climacteric ripening of some important cultivars of banana and development of a dry product from a high carotenoid yielding variety. LWT-Food Science and Technology, 55(1), 59–66. http://dx.doi.org/10.1016/j.lwt.2013.09.005.
  21. Divya, P., Puthusseri Bijesh., & Neelwarne, B. (2012). Carotenoid content, its stability during drying and the antioxidant activity of commercial coriander (Coriandrum sativum L.) varieties. Food Research International, 45(1), 342–350. doi:10.1016/j.foodres.2011.09.021.
  22. Shetty SK, Tiwari N, Marudamuthu AS, Puthusseri Bijesh., Bhandary YP, Fu J, Levin J, Idell S, Shetty S. (2017) p53 and miR-34a Feedback Promotes Lung Epithelial Injury and Pulmonary Fibrosis. The American Journal of Pathology. 187(5), 1016-1034. doi.org/10.1016/j.ajpath.2016.12.020. 
  23. Divya, P., Puthusseri Bijesh., Manual, D. J. K., & Savanur, M. A. (2017). Epigallocatechin gallate protects BEAS-2B cells from lipopolysaccharide-induced apoptosis through upregulation of gastrin-releasing peptide. Molecular and Cellular Biochemistry, 1-7.
  24. Mallappa M, Mohammed Azharuddin S, Shivaraj Y, Puthusseri Bijesh., (2017). Spectroscopic and electrochemical studies on the molecular interaction of copper sulphide nanoparticles with bovine serum albumin. Journal of Materials Science. doi:10.1007/s10853-017-1521-8.

 

Patent:

  1. Dihydrofolate for Hypoglycemic Activity. Vikas Singh Chauhan, Bijesh Puthusseri, Ajana Pathikkal, Ulaganathan Mabalirajan, Atmaja Karmakar, Divya Peethambaran. Application filing date: 31/05/2023, US Patent Application No. 18/326,450
  2. Encapsulated dihydrofolate formulation for dietary supplementation as effective nutraceutical supplement and method of preparation thereof. Bijesh Puthusseri, Vikas Singh Chauhan, Ajana Pathikkal, Ulaganathan Mabalirajan, Sunita Das, Atmaja Karmakar, Divya Peethambaran. Provisional application filing date: 26/03/2024, India 202411023759

 

 

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