Dhabaleswar Patra, Ph.D.

Senior Scientist
Structural Biology & Bioinformatics
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Research Focus

Cardiovascular disease, NAFLD, Neurodegenerative diseases, Signal transduction and Structural Biology (Crystallography & Cryo-EM).

Research Interest

Cardiovascular disease (CVD) is the leading cause of death in the developed as well as in the developing world. The annual mortality of CVD is expected to reach 23.6 million by 2030. Our lab research interests are in understanding molecular mechanisms of cardiovascular disease. Our principal approach is to determine cryo-EM or X-ray structures of proteins involved in such diseases and their downstream signaling components alone and in complex with key-regulators, and then test structure -derived hypotheses via a battery of functional assays. The underlying rationale is that a better understanding of the molecular and cellular mechanisms that underlie the regulation of these proteins will accelerate development of novel therapeutics. A major focus of our lab has been the structure and function of lipid modulating proteins like the PNPLAs and membrane proteins like ABCs, which are dysregulated in chronic disease such as heart failure, non-alcoholic fatty liver disease and neurodegenerative diseases.

The main focus is to study disease biology and empower medicine to achieve a more optimal view towards therapeutic interventions by elucidating the structures and functions of the proteins involved in cardiovascular diseases, NAFLD and neurodegenerative disease. This structural and functional analysis will further help us to promote wellness against some globally alarming diseases like atherosclerosis, liver disease and Alzheimer’s Disease by drug development as a long-term aim.   

Credentials

  •  Senior Scientist (2023-onwards)
  •  Post-doctoral Research Fellow (2018-2022) Purdue University, West Lafayette, Indiana, USA
  •  Post-doctoral Research Fellow (2015-2017) University of Michigan Ann Arbor, Michigan, USA
  •  Ph.D (2014) Indian Institute of Science, Bangalore, India 

Patents & Publications

Publications:

  1. Madasu PK, Maity A, Patra D, Chandran T.  Betacoronaviral lectins: Identification through a genomic search-A structural and evolutionary biology perspective. Journal of Carbohydrate Chemistry 2023 Nov 30. doi: 10.1080/07328303.2023.2285970
  2. Chen Q, Plasencia M, Zhuang Li, Mukherjee S, Patra D, Chen CL, Klose T, Yao X, Chang L, Kossiakof AA, Chang L, Andrews PC, Tesmer JJG. Structure of a Rhodopsin in complex with G-protein-coupled receptor kinase 1. Nature. 2021 Jul 14. doi: 10.1038/s41586-021-03721-x.
  3. Plescia CB, David EA, Patra D, Sengupta R, Amiar S, Su Y, and Stahelin RV. SARS-CoV-2 viral budding and entry can be modeled using BSL-2 level virus-like particles. JBC 2020 Nov 19.  doi: 10.1074/jbc.RA120.016148
  4. Manthei KA, Patra D, Wilson CJ, Fawaz MV, Piersimoni L, Shenkar J, Yuan W, Andrews PC, Engen JR, Schwendeman A, Ohi MD, Tesmer JJG. Structural analysis of lecithin:cholesterol acyltransferase bound to high density lipoprotein particles.  Commun Biol 3, 28 (2020) doi:10.1038/s42003-019-0749-z.  
  5. Beyett TS, Fraley AE, Labudde E, Patra D, Coleman RC, Eguchi A, Glukhova A, Chen Q, Williams RM, Koch WJ, Sherman DH, Tesmer JJG. Perturbation of the interactions of calmodulin with GRK5 using a natural product chemical probe. PNAS. 2019 Aug 6; 116(32):15895-15900 
  6. Seegar TCM, Killingsworth LB, Saha N, Meyer PA, Patra D, Zimmerman B, Janes PW, Rubinstein E, Nikolov DB, Skiniotis G, Kruse AC, Blacklow SC. Structural Basis for Regulated Proteolysis by the α-Secretase ADAM10. Cell. 2017 Dec 14;171(7):1638-1648.
  7. Komolov KE, Du Y, Duc NM, Betz RM, Rodrigues JPGLM, Leib RD, Patra D, Skiniotis G, Adams CM, Dror RO, Chung KY, Kobilka BK, Benovic JL. Structural and Functional Analysis of a β2-Adrenergic Receptor Complex with GRK5. Cell. 2017 Apr 20;169(3):407-421
  8. Das M, Du Y, Ribeiro O, Hariharan P, Mortensen JS, Patra D, Skiniotis G, Loland CJ, Guan L, Kobilka BK, Byrne B, Chae PS. Conformationally preorganized diastereomeric norbornane-based maltosides (NBMs) for membrane protein study: Implications of detergent kink for micellar properties. J Am Chem Soc. 2017 Mar 1;139(8):3072-3081. 
  9. Dasgupta N, Ranjan S, Patra D, Srivastava P, Kumar A, Ramalingam C. Bovine serum albumin interacts with silver nanoparticles with a "side-on" or "end on" conformation. Chem Biol Interact. 2016 253:100-11. 
  10. Patra D, Mishra P, Vijayan M, Surolia A. Negative cooperativity and high affinity in chitooligosaccharide binding by a Mycobacterium smegmatis protein containing LysM and lectin domains. Biochemistry, 2015 55: 49-61. 
  11. Patra D, Mishra P, Surolia A, Vijayan M. Structure, interactions and evolutionary implications of a domain-swapped lectin dimer from Mycobacterium smegmatis. Glycobiology, 2014 24: 956-965.
  12. Patra D, Sharma A, Chandran D, Vijayan M. Cloning, expression, purification, crystallization and preliminary X-ray studies of the mannose- binding lectin domain of MSMEG_3662 from Mycobacterium smegmatis. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2011 67:596-599. 
  13. Patra D, Srikalaivani R, Misra A, Singh DD, Selvaraj M, Vijayan M. Cloning, expression, purification, crystallization and preliminary X-ray studies of a secreted lectin (Rv1419) from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun, 2010 66:1662-1665. 
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