Subrata Adak , Ph.D.

Chief Scientist
Structural Biology & Bioinformatics
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Research Interest

  • The biochemistry of metalloproteins in relation to structure-function relationship and molecular mechanism of catalysis.
  • Oxidative stress responses in Leishmania.

Credentials

PhD, Jadavpur University, 1997 
Fellow of American Heart Association, USA, 2000 
Raman Research Fellow under CSIR, India, 2009-10

Patents & Publications

  1. Chowdhury G, Biswas S, Dholey Y, Panja P, Das S, Adak S. Importance of aspartate 4 in the Mg2+ dependent regulation of Leishmania major PAS domain-containing phosphoglycerate kinase. Biochim Biophys Acta Proteins Proteom. 2023 Sep 17;1872(1):140964. doi: 10.1016/j.bbapap.2023.140964.
  2. Das S, Panja P, Chowdhury G, Biswas S, Dholey Y, Adak S. The ChaC family of γ-glutamyl cyclotransferases is required for Leishmania to switch to a slow growth state and for long-term survival of the parasite. J Biol Chem. 2022 Nov;298(11):102510. doi: 10.1016/j.jbc.2022.102510.
  3. Das P, Mukherjee A, Adak S. Glyceraldehyde-3-phosphate dehydrogenase present in extracellular vesicles from Leishmania major suppresses host TNF-alpha expression. J Biol Chem. 2021 Oct 1;297(4). DOI: 10.1016/j.jbc.2021.101198.
  4. Biswas S, Adhikari A, Mukherjee A, Das S, Adak S. Regulation of Leishmania major PAS domain‐containing phosphoglycerate kinase by cofactor Mg2+ ion at neutral pH. FEBS J. 2020 Dec;287(23):5183-5195. DOI: 10.1111/febs.15305.
  5. Adhikari A, Biswas S, Mukherjee A, Das S, Adak S. PAS domain-containing phosphoglycerate kinase deficiency in Leishmania major results in increased autophagosome formation and cell death. Biochem J. 2019 Apr 30;476(8):1303-1321. DOI: 10.1042/BCJ20190041.
  6. Mukherjee A, Adhikari A, Das P, Biswas S, Mukherjee S, Adak S. Loss of virulence in NAD (P) H cytochrome b5 oxidoreductase deficient Leishmania major. Biochem Biophys Res Commun. 2018 Sep 3;503(1):371-377. DOI: 10.1016/j.bbrc.2018.06.037.
  7. Roy J, Sen Santara S, Adhikari A, Mukherjee A, Adak S. Control of catalysis in globin coupled adenylate cyclase by a globin-B domain. Arch Biochem Biophys. 2015 Aug 1;579:85-90. doi: 10.1016/j.abb.2015.06.007. Epub 2015 Jun 18.
  8. Dolai, S., and Adak, S. Endoplasmic reticulum stress responses in Leishmania (2014) Mol. Biochem. Parasitol. 197, 1-8.
  9. Roy, J., Sen Santara, S., Bose, M., Mukherjee, S., Saha, R., and Adak, S. The ferrous-dioxy complex of Leishmania major globin coupled heme containing adenylate cyclase: The role of proximal histidine on its stability. (2014) Biochim. Biophys. Acta. 1844, 615-622
  10. Saha, R., Bose, M., Santara, S. S., Roy, J. and Adak, S. Identification of proximal and distal axial ligands in Leishmania major pseudoperoxidase (2013) Biochemistry 52, 8878−8887.
  11. Santara, S. S., Roy, J. Mukherjee, S., Bose, M., Saha, R., and Adak, S. Globin coupled heme containing oxygen sensor soluble adenylate cyclase in Leishmania prevents cell death during hypoxia (2013) Proc. Natl. Acad. Sci. U.S.A. 110, 16790–16795
  12. Adak, S. and Pal, S.: Ascorbate peroxidase acts as a novel determiner of redox homeostasis in Leishmania. (2013) Antioxid Redox Signal. 19, 746-754
  13. Saha, R., Bose, M., Santara, S. S., Roy, J. Yadav, R.K., and Adak, S. Effect of distal His mutation on the peroxynitrite reactivity of Leishmania major peroxidase (2013) Biochim. Biophys. Acta. 1834, 2057-2063
  14. Saha, R., Bose, M. and Adak, S. Mutation of Val90 to His in the pseudoperoxidase from Leishmania major enhances peroxidase activity (2013) Biochim. Biophys. Acta. 1834, 651-657
  15. Mukherjee, S., Santara, S. S., Das, S., Bose, M., Roy, J. and Adak, S. NAD(P)H cytochrome b5 oxidoreductase deficiency in Leishmania major results in impaired linoleate synthesis followed by increased oxidative stress and cell death. (2012) J. Biol. Chem. 287, 34992-35003
  16. Bose, M., Saha, R., Santara, S. S., Mukherjee, S., Roy, J. and Adak, S. Protection against peroxynitrite by pseudoperoxidase from Leishmania major. (2012) Free. Radic. Biol. Med. 53, 1819–1828
  17. Pal, S., Yadav, R.K. and Adak S.: Role of K+ binding residues in stabilization of heme spin state of Leishmania major peroxidase. (2012) Biochim. Biophys. Acta. 1824, 1002–1007
  18. Dolai, S., Pal, S., Yadav, R.K. and Adak S. Endoplasmic reticulum stress-induced apoptosis in Leishmania through Ca2+-dependent and caspase-independent mechanism (2011) J. Biol. Chem. 286, 13638-13646
  19. Yadav, R.K., Pal, S., Dolai, S. and Adak S.: Role of proximal methionine residues in Leishmania major peroxidase. (2011) Arch. Biochem. Biophys. 515, 21–27. Cover page article.
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